A Ribosome-Inactivating Protein from Amaranthus viridis

Seok Yoon Kwon, Chung Sun An, Jang Ryol Liu, Kyung Hee Paek

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

An antiviral protein purified from the leaves of Amaranthus viridis was named amaranthin. The in vivo antiviral activity of amaranthin was confirmed in tobacco mosaic virus (TMV) infection test on Nicotiana glutinosa leaves. The molecular mass of the amaranthin was estimated about 30 kDa by SDS-PAGE and the pI was measured as 9.8 by isoelectric focusing (IEF) analysis. Cytotoxicity of the amaranthin using in vitro translation inhibition assay was similar to that of pokeweed antiviral protein (PAP) with IC50 of 25 pM. Depurination activity (N-glycosidase activity) against animal rRNA was also confirmed.

Original languageEnglish
Pages (from-to)1613-1614
Number of pages2
JournalBioscience, Biotechnology and Biochemistry
Volume61
Issue number9
Publication statusPublished - 1997 Sep 1

Fingerprint

Amaranthus viridis
antiviral proteins
Ribosome Inactivating Proteins
Amaranthus
ribosomes
Antiviral Agents
Nicotiana glutinosa
Proteins
Tobacco Mosaic Virus
Phytolacca americana
Tobacco mosaic virus
Tobacco
glycosidases
Glycoside Hydrolases
isoelectric focusing
Isoelectric Focusing
Molecular mass
Virus Diseases
Cytotoxicity
Viruses

Keywords

  • Amaranthus viridis
  • Antiviral protein
  • In vitro translation
  • RIP
  • TMV

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

A Ribosome-Inactivating Protein from Amaranthus viridis. / Kwon, Seok Yoon; An, Chung Sun; Liu, Jang Ryol; Paek, Kyung Hee.

In: Bioscience, Biotechnology and Biochemistry, Vol. 61, No. 9, 01.09.1997, p. 1613-1614.

Research output: Contribution to journalArticle

Kwon, Seok Yoon ; An, Chung Sun ; Liu, Jang Ryol ; Paek, Kyung Hee. / A Ribosome-Inactivating Protein from Amaranthus viridis. In: Bioscience, Biotechnology and Biochemistry. 1997 ; Vol. 61, No. 9. pp. 1613-1614.
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