A Ribosome–Inactivating Protein from Amaranthus viridis >)

Seok Yoon Kwon, Chung Sun An, Jang Ryol Liu, Kyung Hee Paek

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

An antiviral protein purified from the leaves of Amaranthus viridis was named amaranthin. The in vivo antiviral activity of amaranthin was confirmed in tobacco mosaic virus (TMV) infection test on Nicotiana glutinosa leaves. The molecular mass of the amaranthin was estimated about 30 kDa by SDS–PAGE and the pI was measured as 9.8 by isoelectric focusing (IEF) analysis. Cytotoxicity of the amaranthin using in vitro translation inhibition assay was similar to that of pokeweed antiviral protein (PAP) with IC50 of 25pM. Depurination activity (N-glycosidase activity) against animal rRNA was also confirmed.

Original languageEnglish
Pages (from-to)1613-1614
Number of pages2
JournalBioscience, Biotechnology and Biochemistry
Volume61
Issue number9
DOIs
Publication statusPublished - 1997 Jan

Keywords

  • Amaranthus viridis
  • Antiviral protein
  • In vitro translation
  • RIP
  • TMV

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'A Ribosome–Inactivating Protein from Amaranthus viridis >)'. Together they form a unique fingerprint.

  • Cite this