A rice orthologue of the ABA receptor, OsPYL/RCAR5, is a positive regulator of the ABA signal transduction pathway in seed germination and early seedling growth

Hyunmi Kim, Hyunsik Hwang, Jung Woo Hong, Young Na Lee, Il Pyung Ahn, In Sun Yoon, Sang Dong Yoo, Sukchan Lee, Sung Chul Lee, Beom Gi Kim

Research output: Contribution to journalArticlepeer-review

147 Citations (Scopus)

Abstract

Abscisic acid (ABA) is a phytohormone that positively regulates seed dormancy and stress tolerance. PYL/RCARs were identified an intracellular ABA receptors regulating ABA-dependent gene expression in Arabidopsis thaliana. However, their function in monocot species has not been characterized yet. Herein, it is demonstrated that PYL/RCAR orthologues in Oryza sativa function as a positive regulator of the ABA signal transduction pathway. Transgenic rice plants expressing OsPYL/RCAR5, a PYL/RCAR orthologue of rice, were found to be hypersensitive to ABA during seed germination and early seedling growth. A rice ABA signalling unit composed of OsPYL/RCAR5, OsPP2C30, SAPK2, and OREB1 for ABA-dependent gene regulation was further identified, via interaction assays and a transient gene expression assay. Thus, a core signalling unit for ABA-responsive gene expression modulating seed germination and early seedling growth in rice has been unravelled. This study provides substantial contributions toward understanding the ABA signal transduction pathway in rice.

Original languageEnglish
Pages (from-to)1013-1024
Number of pages12
JournalJournal of experimental botany
Volume63
Issue number2
DOIs
Publication statusPublished - 2012 Jan

Keywords

  • ABA receptor
  • PP2C
  • SnRK2
  • rice

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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