A role for the purinergic receptor P2X3 in astrocytes in the mechanism of craniofacial neuropathic pain

Won Mah, Sang Man Lee, Jaekwang Lee, Jin Young Bae, Jin Sook Ju, Changjoon Lee, Dong Kuk Ahn, Yong Chul Bae

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Abstract

The purinergic receptor P2X3, expressed in the central terminals of primary nociceptive neurons in the brainstem, plays an important role in pathological pain. However, little is known about expression of P2X3 in the brainstem astrocytes and its involvement in craniofacial pathologic pain. To address this issue, we investigated the expression of P2X3 in astrocytes in the trigeminal caudal nucleus (Vc) in a rat model of craniofacial neuropathic pain, chronic constriction injury of infraorbital nerve (CCI-ION). We found that 1) P2X3-immunoreactivity is observed in the brainstem astrocytes, preferentially in their fine processes, 2) the number of P2X3-positive fine astrocytic processes and the density of P2X3 in these processes were increased significantly in CCI-ION rats, compared to control rats, and 3) administration of MPEP, a specific mGluR5 antagonist, alleviated the mechanical allodynia and abolished the increase in density of P2X3 in fine astrocytic processes caused by CCI-ION. These findings reveal preferential expression of P2X3 in the fine astrocytic processes in the brainstem, propose a novel role of P2X3 in the fine astrocytic process in the mechanism of craniofacial neuropathic pain, and suggest that the expression of astrocytic P2X3 may be regulated by astrocytic mGluR5.

Original languageEnglish
Article number13627
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1
Externally publishedYes

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Purinergic P2X3 Receptors
Facial Pain
Neuralgia
Astrocytes
Brain Stem
Constriction
Trigeminal Caudal Nucleus
Wounds and Injuries
Nociceptors
Hyperalgesia
Pain

ASJC Scopus subject areas

  • General

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A role for the purinergic receptor P2X3 in astrocytes in the mechanism of craniofacial neuropathic pain. / Mah, Won; Lee, Sang Man; Lee, Jaekwang; Bae, Jin Young; Ju, Jin Sook; Lee, Changjoon; Ahn, Dong Kuk; Bae, Yong Chul.

In: Scientific Reports, Vol. 7, No. 1, 13627, 01.12.2017.

Research output: Contribution to journalArticle

Mah, Won ; Lee, Sang Man ; Lee, Jaekwang ; Bae, Jin Young ; Ju, Jin Sook ; Lee, Changjoon ; Ahn, Dong Kuk ; Bae, Yong Chul. / A role for the purinergic receptor P2X3 in astrocytes in the mechanism of craniofacial neuropathic pain. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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