A rule governing the FtsH-mediated proteolysis of the MgtC virulence protein from Salmonella enterica serovar Typhimurium

Jonghyun Baek, Eunna Choi, Eun-Jin Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A tightly controlled turnover of membrane proteins is required for lipid bilayer stability, cell metabolism, and cell viability. Among the energy-dependent AAA+ proteases in Salmonella, FtsH is the only membrane-bound protease that contributes to the quality control of membrane proteins. FtsH preferentially degrades the C-terminus or N-terminus of misfolded, misassembled, or damaged proteins to maintain physiological functions. We found that FtsH hydrolyzes the Salmonella MgtC virulence protein when we substitute the MgtC 226th Trp, which is well conserved in other intracellular pathogens and normally protects MgtC from the FtsH-mediated proteolysis. Here we investigate a rule determining the FtsH-mediated proteolysis of the MgtC protein at Trp226 residue. Substitution of MgtC tryptophan 226th residue to alanine, glycine, or tyrosine leads to MgtC proteolysis in a manner dependent on the FtsH protease whereas substitution to phenylalanine, methionine, isoleucine, leucine, or valine resists MgtC degradation by FtsH. These data indicate that a large and hydrophobic side chain at 226th residue is required for protection from the FtsH-mediated MgtC proteolysis.

Original languageEnglish
Pages (from-to)565-570
Number of pages6
JournalJournal of Microbiology
Volume56
Issue number8
DOIs
Publication statusPublished - 2018 Aug 1
Externally publishedYes

Fingerprint

Salmonella enterica
Proteolysis
Virulence
Peptide Hydrolases
Salmonella
Membrane Proteins
Proteins
Isoleucine
Valine
Lipid Bilayers
Phenylalanine
Leucine
Tryptophan
Quality Control
Alanine
Methionine
Glycine
Tyrosine
Cell Survival
Membranes

Keywords

  • FtsH protease
  • mgtC
  • post-translational regulation

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

A rule governing the FtsH-mediated proteolysis of the MgtC virulence protein from Salmonella enterica serovar Typhimurium. / Baek, Jonghyun; Choi, Eunna; Lee, Eun-Jin.

In: Journal of Microbiology, Vol. 56, No. 8, 01.08.2018, p. 565-570.

Research output: Contribution to journalArticle

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