A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay

Hye Jin Lee, Byoung Chan Kim, Min Kyu Oh, Jungbae Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Stable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 °C for 66 days.

Original languageEnglish
Pages (from-to)5971-5973
Number of pages3
JournalChemical Communications
Volume48
Issue number48
DOIs
Publication statusPublished - 2012 May 21

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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