A sensitive and reliable detection of thrombin via enzyme-precipitate- coating-linked aptamer assay

Hye Jin Lee, Byoung Chan Kim, Min-Kyu Oh, Jungbae Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Stable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 °C for 66 days.

Original languageEnglish
Pages (from-to)5971-5973
Number of pages3
JournalChemical Communications
Volume48
Issue number48
DOIs
Publication statusPublished - 2012 Jun 18

Fingerprint

Thrombin
Precipitates
Assays
Enzymes
Coatings
Signal detection
Limit of Detection

ASJC Scopus subject areas

  • Metals and Alloys
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Catalysis
  • Chemistry(all)

Cite this

A sensitive and reliable detection of thrombin via enzyme-precipitate- coating-linked aptamer assay. / Lee, Hye Jin; Kim, Byoung Chan; Oh, Min-Kyu; Kim, Jungbae.

In: Chemical Communications, Vol. 48, No. 48, 18.06.2012, p. 5971-5973.

Research output: Contribution to journalArticle

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