A Sexually Transmitted Disease (STD) DNA chip for the diagnosis of genitourinary infections

Won young Chung, Ye Lim Jung, Ki Soo Park, Cheulhee Jung, Sung Chul Shin, Sang Joon Hwang, Dae Yeon Cho, Sun Ho Cha, Si Kyu Lim, Hyun Gyu Park

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The results of an investigation aimed at the development of a DNA chip for the detection of genitourinary infections are described. Through analysis of over 35,000 clinical cases, 14 pathogens which are most abundantly found among Koreans were selected and candidate sequences for capture probes were accordingly chosen by considering their sequences and β-globin house-keeping gene. Among this group, the most suitable capture probe sequences were selected by employing repeated chip tests in which they are immobilized on a glass chip by using a recently developed novel gold nanoparticles-based method. A multiplex PCR method was established to generate fluorescence-labeled sequences for all 14 pathogens along with the β-globin gene. By using optimized hybridization conditions, the final chip was constructed and employed to diagnose reliably both single and multiple infections in clinical human samples for 14 target pathogens. The results show that the novel chip methodology serves as a highly reliable and convenient tool for the diagnosis of Sexually Transmitted Diseases (STDs). Furthermore, this study has its great significance in that it demonstrates the entire process from statistical analysis of a large number of clinical cases to the final development of STD DNA chip just ready to be applied or commercialized in the clinical diagnostic field.

Original languageEnglish
Pages (from-to)4314-4319
Number of pages6
JournalBiosensors and Bioelectronics
Volume26
Issue number11
DOIs
Publication statusPublished - 2011 Jul 15

Keywords

  • DNA chip
  • Genitourinary infection
  • Multiplex PCR
  • STD pathogen

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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  • Cite this

    Chung, W. Y., Jung, Y. L., Park, K. S., Jung, C., Shin, S. C., Hwang, S. J., Cho, D. Y., Cha, S. H., Lim, S. K., & Park, H. G. (2011). A Sexually Transmitted Disease (STD) DNA chip for the diagnosis of genitourinary infections. Biosensors and Bioelectronics, 26(11), 4314-4319. https://doi.org/10.1016/j.bios.2011.04.024