A shape-code nanoplasmonic biosensor for multiplex detection of Alzheimer's disease biomarkers

Hanbi Kim, Jong Uk Lee, Sojin Song, Soohyun Kim, Sang Jun Sim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Alzheimer's disease (AD) is a neurodegenerative disease associated with the loss of nerve cells in the brain. The disease is affected by multifactorial pathways and leads to changes in related biomolecular levels as AD progresses. Therefore, AD should be diagnosed with combined detection of several lesions to improve accuracy. Amyloid beta 1–40, 1–42 and τ (tau) protein are milestones in AD pathology and can be used as main screening and diagnostic target markers. Here, we suggest a highly selective biosensor for detection of AD core biomarkers on one platform through distinct localized surface plasmon resonance (LSPR) depending on gold nanoparticles shapes, called a shape-code biosensor. This plasmonic sensor consists of only gold nanoparticles and antibody, but does not need additory methods for precise separation from multifarious samples and identification of markers. Under physiological condition, we determined a detection limit of 34.9 fM for amyloid beta (Aβ) 1–40, 26 fM for Aβ 1–42 and 23.6 fM for τ protein corresponding to the ~ 1, ~ 2.23 and ~ 3.12 nm of Rayleigh scattering peak shift on shape-code plasmon system for each biomarker in mimicked blood. This is the first highly sensitive shape-code biosensor to detect AD biomarkers which can be used to diagnose AD easily in the future.

Original languageEnglish
Pages (from-to)96-102
Number of pages7
JournalBiosensors and Bioelectronics
Volume101
DOIs
Publication statusPublished - 2018 Mar 15

Fingerprint

Biosensing Techniques
Biomarkers
Biosensors
Alzheimer Disease
Amyloid
Gold
Nanoparticles
tau Proteins
Neurodegenerative diseases
Proteins
Surface Plasmon Resonance
Rayleigh scattering
Neurodegenerative Diseases
Surface plasmon resonance
Pathology
Limit of Detection
Antibodies
Neurons
Brain
Screening

Keywords

  • Alzheimer's disease (AD)
  • Gold nanoparticles (AuNPs)
  • Localized surface plasmon resonance (LSPR)
  • Multiplex detection
  • Plasmonic biosensors
  • Shape-code

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

A shape-code nanoplasmonic biosensor for multiplex detection of Alzheimer's disease biomarkers. / Kim, Hanbi; Lee, Jong Uk; Song, Sojin; Kim, Soohyun; Sim, Sang Jun.

In: Biosensors and Bioelectronics, Vol. 101, 15.03.2018, p. 96-102.

Research output: Contribution to journalArticle

Kim, Hanbi ; Lee, Jong Uk ; Song, Sojin ; Kim, Soohyun ; Sim, Sang Jun. / A shape-code nanoplasmonic biosensor for multiplex detection of Alzheimer's disease biomarkers. In: Biosensors and Bioelectronics. 2018 ; Vol. 101. pp. 96-102.
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