TY - JOUR
T1 - A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers using PVP
AU - Kim, C. S.
AU - Lee, C. H.
AU - Shin, J. S.
AU - Chung, Y. S.
AU - Hyung, N. I.
PY - 1996/9/5
Y1 - 1996/9/5
N2 - Because DNA degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality DNA from plants containing a high content of polyphenolics has been a difficult problem. We demonstrate an easy extraction process by modifying several existing ones. Using this process we have found it possible to isolate DNAs from four fruit trees, grape (Vitis spp.), apple (Malus spp.), pear (Pyrus spp.) and persimmon (Diospyros spp.) and four species of conifer, Pinus densiflora, Pinus koraiensis, Taxus cuspidata and Juniperus chinensis within a few hours. Compared with the existing method, we have isolated high quality intact DNAs (260/280 = 1.8-2.0) routinely yielding 250-500 ng/μl (total 7.5-15 μg DNA from four to five tissue discs).
AB - Because DNA degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality DNA from plants containing a high content of polyphenolics has been a difficult problem. We demonstrate an easy extraction process by modifying several existing ones. Using this process we have found it possible to isolate DNAs from four fruit trees, grape (Vitis spp.), apple (Malus spp.), pear (Pyrus spp.) and persimmon (Diospyros spp.) and four species of conifer, Pinus densiflora, Pinus koraiensis, Taxus cuspidata and Juniperus chinensis within a few hours. Compared with the existing method, we have isolated high quality intact DNAs (260/280 = 1.8-2.0) routinely yielding 250-500 ng/μl (total 7.5-15 μg DNA from four to five tissue discs).
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U2 - 10.1093/nar/25.5.1085
DO - 10.1093/nar/25.5.1085
M3 - Article
C2 - 9023124
AN - SCOPUS:0030802379
VL - 25
SP - 1085
EP - 1086
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 5
ER -