A simple and reliable method for obtaining Entomosporium monoconidial isolates

Mi Jeong Park, Quinn A. Holtslag, Hyeon-Dong Shin

Research output: Contribution to journalArticle

Abstract

Monoconidial isolates of Entomosporium mespili were successfully cultured using a simple isolation procedure. A detailed description of the steps required for isolating E. mespili is provided. The characteristic pattern of conidial germination and growth on potato dextrose agar is also described. The process that was successful in obtaining pure isolates involved collecting material in the morning, picking a glistening white mass of conidia under dissecting microscope magnification, depositing a mass of conidia onto a drop of water on a glass slide, streaking the conidial mass onto potato dextrose agar, incubating the plate for 48 h, and transferring the characteristic fan-shaped colonies to fresh medium.

Original languageEnglish
Pages (from-to)324-326
Number of pages3
JournalJournal of Microbiology
Volume49
Issue number2
DOIs
Publication statusPublished - 2011 Apr 1

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Fungal Spores
Solanum tuberosum
Agar
Glucose
Germination
Glass
Water
Growth

Keywords

  • Entomosporium mespili
  • fan-shaped colony
  • germination
  • isolation technique

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

A simple and reliable method for obtaining Entomosporium monoconidial isolates. / Park, Mi Jeong; Holtslag, Quinn A.; Shin, Hyeon-Dong.

In: Journal of Microbiology, Vol. 49, No. 2, 01.04.2011, p. 324-326.

Research output: Contribution to journalArticle

Park, Mi Jeong ; Holtslag, Quinn A. ; Shin, Hyeon-Dong. / A simple and reliable method for obtaining Entomosporium monoconidial isolates. In: Journal of Microbiology. 2011 ; Vol. 49, No. 2. pp. 324-326.
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