A simple dual online ultra-high pressure liquid chromatography system (sDO-UHPLC) for high throughput proteome analysis

Hangyeore Lee, Dong Gi Mun, Jingi Bae, Hokeun Kim, Se Yeon Oh, Young Soo Park, Jae Hyuk Lee, Sang-Won Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We report a new and simple design of a fully automated dual-online ultra-high pressure liquid chromatography system. The system employs only two nano-volume switching valves (a two-position four port valve and a two-position ten port valve) that direct solvent flows from two binary nano-pumps for parallel operation of two analytical columns and two solid phase extraction (SPE) columns. Despite the simple design, the sDO-UHPLC offers many advantageous features that include high duty cycle, back flushing sample injection for fast and narrow zone sample injection, online desalting, high separation resolution and high intra/inter-column reproducibility. This system was applied to analyze proteome samples not only in high throughput deep proteome profiling experiments but also in high throughput MRM experiments.

Original languageEnglish
Pages (from-to)5700-5706
Number of pages7
JournalAnalyst
Volume140
Issue number16
DOIs
Publication statusPublished - 2015 Aug 21

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High pressure liquid chromatography
Proteome
liquid chromatography
High Pressure Liquid Chromatography
Throughput
Proteins
Salt removal
Injections
Solid Phase Extraction
Experiments
Pumps
flushing
pump
experiment
analysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry

Cite this

A simple dual online ultra-high pressure liquid chromatography system (sDO-UHPLC) for high throughput proteome analysis. / Lee, Hangyeore; Mun, Dong Gi; Bae, Jingi; Kim, Hokeun; Oh, Se Yeon; Park, Young Soo; Lee, Jae Hyuk; Lee, Sang-Won.

In: Analyst, Vol. 140, No. 16, 21.08.2015, p. 5700-5706.

Research output: Contribution to journalArticle

Lee, Hangyeore ; Mun, Dong Gi ; Bae, Jingi ; Kim, Hokeun ; Oh, Se Yeon ; Park, Young Soo ; Lee, Jae Hyuk ; Lee, Sang-Won. / A simple dual online ultra-high pressure liquid chromatography system (sDO-UHPLC) for high throughput proteome analysis. In: Analyst. 2015 ; Vol. 140, No. 16. pp. 5700-5706.
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