A simple method for constructing a tagged protein

C. Jo, J. Kim, S. A. Jo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day.

Original languageEnglish
Pages (from-to)631-633
Number of pages3
JournalProtein and Peptide Letters
Volume12
Issue number7
DOIs
Publication statusPublished - 2005 Oct

Keywords

  • Affinity tag
  • Gene fusion
  • Polymerase chain reaction (PCR)
  • Recombinant protein
  • Small ubiquitin-related modifier (SUMO)-1

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry

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