A simple method for constructing a tagged protein

C. Jo, Joon Kim, S. A. Jo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day.

Original languageEnglish
Pages (from-to)631-633
Number of pages3
JournalProtein and Peptide Letters
Volume12
Issue number7
DOIs
Publication statusPublished - 2005 Oct 1

Fingerprint

His-His-His-His-His-His
Polymerase Chain Reaction
DNA
Ligation
Proteins
Genes

Keywords

  • Affinity tag
  • Gene fusion
  • Polymerase chain reaction (PCR)
  • Recombinant protein
  • Small ubiquitin-related modifier (SUMO)-1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

A simple method for constructing a tagged protein. / Jo, C.; Kim, Joon; Jo, S. A.

In: Protein and Peptide Letters, Vol. 12, No. 7, 01.10.2005, p. 631-633.

Research output: Contribution to journalArticle

Jo, C. ; Kim, Joon ; Jo, S. A. / A simple method for constructing a tagged protein. In: Protein and Peptide Letters. 2005 ; Vol. 12, No. 7. pp. 631-633.
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