A simple method for constructing a tagged protein

C. Jo; J. Kim; S. A. Jo

doi:10.2174/0929866054696064

A simple method for constructing a tagged protein

C. Jo, J. Kim, S. A. Jo

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His₆ tag sequence with high efficiency by the next day.

Original language	English
Pages (from-to)	631-633
Number of pages	3
Journal	Protein and Peptide Letters
Volume	12
Issue number	7
DOIs	https://doi.org/10.2174/0929866054696064
Publication status	Published - 2005 Oct

Keywords

Affinity tag
Gene fusion
Polymerase chain reaction (PCR)
Recombinant protein
Small ubiquitin-related modifier (SUMO)-1

ASJC Scopus subject areas

Structural Biology
Biochemistry

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10.2174/0929866054696064

Cite this

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title = "A simple method for constructing a tagged protein",

abstract = "We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day.",

keywords = "Affinity tag, Gene fusion, Polymerase chain reaction (PCR), Recombinant protein, Small ubiquitin-related modifier (SUMO)-1",

author = "C. Jo and J. Kim and Jo, {S. A.}",

year = "2005",

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AU - Jo, S. A.

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AB - We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day.

KW - Affinity tag

KW - Gene fusion

KW - Polymerase chain reaction (PCR)

KW - Recombinant protein

KW - Small ubiquitin-related modifier (SUMO)-1

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A simple method for constructing a tagged protein

Abstract

Keywords

ASJC Scopus subject areas

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