We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day.
- Affinity tag
- Gene fusion
- Polymerase chain reaction (PCR)
- Recombinant protein
- Small ubiquitin-related modifier (SUMO)-1
ASJC Scopus subject areas
- Structural Biology