A simple protocol to convert sitting-drop vapor-diffusion plating into a hanging-drop vapor-diffusion experiment in protein crystallization is reported. After making a sitting-drop plate, agarose solution was added to solidify the reservoir solution, and the plates were incubated upside down. Crystallization experiments with hen egg white lysozyme, thaumatin and glucose isomerase showed that the upside-down sitting-drop method could produce single crystals with all the benefits of the hanging-drop crystallization method.
- Agarose gel
- Glucose isomerase
- Protein crystallography
- Solidified reservoirs
- Upside-down sitting-drop vapor diffusion
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)