A simple technique to convert sitting-drop vapor diffusion into hanging-drop vapor diffusion by solidifying the reservoir solution with agarose

Tae Woong Whon, Yong Hwan Lee, Dong Shan An, Hyun Kyu Song, Song G. Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A simple protocol to convert sitting-drop vapor-diffusion plating into a hanging-drop vapor-diffusion experiment in protein crystallization is reported. After making a sitting-drop plate, agarose solution was added to solidify the reservoir solution, and the plates were incubated upside down. Crystallization experiments with hen egg white lysozyme, thaumatin and glucose isomerase showed that the upside-down sitting-drop method could produce single crystals with all the benefits of the hanging-drop crystallization method.

Original languageEnglish
Pages (from-to)975-976
Number of pages2
JournalJournal of Applied Crystallography
Volume42
Issue number5
DOIs
Publication statusPublished - 2009 Sep 28

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Keywords

  • Agarose gel
  • Crystallization
  • Glucose isomerase
  • Lysozyme
  • Protein crystallography
  • Solidified reservoirs
  • Thaumatin
  • Upside-down sitting-drop vapor diffusion

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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