A single administration of 2,3,7,8-tetrachlorodibenzo-p-dioxin that produces reduced food and water intake induces long-lasting expression of corticotropin-releasing factor, arginine vasopressin, and proopiomelanocortin in rat brain

Bo Hyun Moon, Chang Gwun Hong, Soo Young Kim, Hyun Ju Kim, Seung Keon Shin, Seungwoo Kang, Kuem Ju Lee, Yong Ku Kim, Min Soo Lee, Kyung Ho Shin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The mechanism by which a single administration of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) reduces food and water intake is unclear. We examined whether such a food and water intake-reducing single administration of TCDD induced changes in corticotropin-releasing factor (CRF), arginine vasopressin (AVP), and proopiomelanocortin (POMC) expression in rat brain. To observe time-dependent changes in these neuropeptides, male Sprague-Dawley rats were given TCDD (50 μg/kg) and terminated 1, 2, 4, or 7 days later. In addition, to observe dose-dependent changes in feeding and neuropeptides, rats were also given a range of TCDD doses (12.5, 25, or 50 μg/kg) and terminated 14 days later. TCDD suppressed food and water intake over 14 days in a dose-dependent manner. TCDD treatment also increased CRF and POMC mRNA levels in the hypothalamic paraventricular nucleus (PVN) and arcuate nucleus, respectively, in a dose- and time-dependent manner. These increases were related to decreased food intake following TCDD administration. TCDD treatment increased AVP and CRF mRNA levels in the PVN, and these increases were related to decreased water intake. Interestingly, the increases in CRF, AVP and POMC expression were observed 7 to 14 days after TCDD administration. These results suggest that a single administration of TCDD induced long-lasting increases in CRF, AVP, and POMC mRNA levels in the hypothalamus and that these changes are related to reduced food and water intake 7 to 14 days after TCDD administration.

Original languageEnglish
Pages (from-to)314-322
Number of pages9
JournalToxicology and Applied Pharmacology
Volume233
Issue number2
DOIs
Publication statusPublished - 2008 Dec 1

Keywords

  • 2,3,7,8-tetrachlorodibenzo-p-dioxin
  • Arginine vasopressin
  • Corticotropin-releasing factor
  • Proopiomelanocortin

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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