@article{5c34717ff39840e0897e94b36977eb21,
title = "A sleep state in Drosophila larvae required for neural stem cell proliferation",
abstract = "Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larvae, but dopamine is not required. Using real-time behavioral monitoring in a closed-loop sleep deprivation system, we find that sleep loss in larvae impairs cell division of neural progenitors. This work establishes a system uniquely suited for studying sleep during nascent periods, and demonstrates that sleep in early life regulates neural stem cell proliferation.",
author = "Milan Szuperak and Churgin, {Matthew A.} and Borja, {Austin J.} and Raizen, {David M.} and Christopher Fang-Yen and Kayser, {Matthew S.}",
note = "Funding Information: We thank members of the Kayser Lab and Dr. Amita Sehgal for helpful discussions. This work was supported by NIH grants K08NS090461 (MSK), R01NS088432 (DMR and CF-Y), and R01NS084835 (CF-Y), and a Burroughs Wellcome Career Award for Medical Scientists, March of Dimes Basil O{\textquoteright}Connor Scholar Award, and Sloan Research Fellowship (MSK). MS designed and performed experiments, analyzed data, and contributed to writing the manuscript. MAC developed the Larva- Lodge, designed experiments, wrote closed-loop deprivation and analysis software, and provided technical assistance. AJB designed and performed experiments, and analyzed data. DMR designed experiments and interpreted data. CF-Y developed the LarvaLodge, provided technical assistance, and interpreted data. MSK conceived and analyzed experiments, interpreted the data, and wrote the manuscript with input from other authors. National Institutes of Health K08NS090461 Matthew S Kayser Burroughs Wellcome Fund Matthew S Kayser Alfred P. Sloan Foundation Matthew S Kayser March of Dimes Foundation Matthew S Kayser National Institutes of Health R01NS088432 David M Raizen National Institutes of Health R01NS084835 Christopher Fang-Yen The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Funding Information: We thank members of the Kayser Lab and Dr. Amita Sehgal for helpful discussions. This work was supported by NIH grants K08NS090461 (MSK), R01NS088432 (DMR and CF-Y), and R01NS084835 (CF-Y), and a Burroughs Wellcome Career Award for Medical Scientists, March of Dimes Basil O{\textquoteright}Connor Scholar Award, and Sloan Research Fellowship (MSK). MS designed and performed experiments, analyzed data, and contributed to writing the manuscript. MAC developed the Larva-Lodge, designed experiments, wrote closed-loop deprivation and analysis software, and provided technical assistance. AJB designed and performed experiments, and analyzed data. DMR designed experiments and interpreted data. CF-Y developed the LarvaLodge, provided technical assistance, and interpreted data. MSK conceived and analyzed experiments, interpreted the data, and wrote the manuscript with input from other authors. Publisher Copyright: {\textcopyright} Szuperak et al.",
year = "2018",
month = feb,
day = "9",
doi = "10.7554/eLife.33220",
language = "English",
volume = "7",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}