A sleep state in Drosophila larvae required for neural stem cell proliferation

Milan Szuperak, Matthew A. Churgin, Austin J. Borja, David M. Raizen, Christopher Fang-Yen, Matthew S. Kayser

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larvae, but dopamine is not required. Using real-time behavioral monitoring in a closed-loop sleep deprivation system, we find that sleep loss in larvae impairs cell division of neural progenitors. This work establishes a system uniquely suited for studying sleep during nascent periods, and demonstrates that sleep in early life regulates neural stem cell proliferation.

Original languageEnglish
Article numbere33220
JournaleLife
Volume7
DOIs
Publication statusPublished - 2018 Feb 9
Externally publishedYes

Fingerprint

Neural Stem Cells
Cell proliferation
Stem cells
Drosophila
Larva
Sleep
Cell Proliferation
Octopamine
Sleep Deprivation
Neurogenesis
Arousal
Neurology
Cell Division
Nervous System
Neurotransmitter Agents
Dopamine
Norepinephrine
Refining
Neurons
Brain

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Szuperak, M., Churgin, M. A., Borja, A. J., Raizen, D. M., Fang-Yen, C., & Kayser, M. S. (2018). A sleep state in Drosophila larvae required for neural stem cell proliferation. eLife, 7, [e33220]. https://doi.org/10.7554/eLife.33220

A sleep state in Drosophila larvae required for neural stem cell proliferation. / Szuperak, Milan; Churgin, Matthew A.; Borja, Austin J.; Raizen, David M.; Fang-Yen, Christopher; Kayser, Matthew S.

In: eLife, Vol. 7, e33220, 09.02.2018.

Research output: Contribution to journalArticle

Szuperak, M, Churgin, MA, Borja, AJ, Raizen, DM, Fang-Yen, C & Kayser, MS 2018, 'A sleep state in Drosophila larvae required for neural stem cell proliferation', eLife, vol. 7, e33220. https://doi.org/10.7554/eLife.33220
Szuperak M, Churgin MA, Borja AJ, Raizen DM, Fang-Yen C, Kayser MS. A sleep state in Drosophila larvae required for neural stem cell proliferation. eLife. 2018 Feb 9;7. e33220. https://doi.org/10.7554/eLife.33220
Szuperak, Milan ; Churgin, Matthew A. ; Borja, Austin J. ; Raizen, David M. ; Fang-Yen, Christopher ; Kayser, Matthew S. / A sleep state in Drosophila larvae required for neural stem cell proliferation. In: eLife. 2018 ; Vol. 7.
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