A Small-Molecule Probe for Selective Profiling and Imaging of Monoamine Oxidase B Activities in Models of Parkinson's Disease

Lin Li, Cheng Wu Zhang, Jingyan Ge, Linghui Qian, Bing Han Chai, Qing Zhu, Jun Seok Lee, Kah Leong Lim, Shao Q. Yao

Research output: Contribution to journalArticlepeer-review

72 Citations (Scopus)

Abstract

The design of the first dual-purpose activity-based probe of monoamine oxidase B (MAO-B) is reported. This probe is highly selective towards MAO-B, even at high MAO-A expression levels, and could sensitively report endogenous MAO-B activities by both in situ proteome profiling and live-cell bioimaging. With a built-in imaging module as part of the probe design, the probe was able to accomplish what all previously reported MAO-B imaging probes failed to do thus far: the live-cell imaging of MAO-B activities without encountering diffusion problems.

Original languageEnglish
Pages (from-to)10821-10825
Number of pages5
JournalAngewandte Chemie - International Edition
Volume54
Issue number37
DOIs
Publication statusPublished - 2015 Sep 1
Externally publishedYes

Keywords

  • activity-based probes
  • imaging
  • monoamine oxidases
  • Parkinson's disease
  • proteomics

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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