The design of the first dual-purpose activity-based probe of monoamine oxidase B (MAO-B) is reported. This probe is highly selective towards MAO-B, even at high MAO-A expression levels, and could sensitively report endogenous MAO-B activities by both in situ proteome profiling and live-cell bioimaging. With a built-in imaging module as part of the probe design, the probe was able to accomplish what all previously reported MAO-B imaging probes failed to do thus far: the live-cell imaging of MAO-B activities without encountering diffusion problems.
|Number of pages||5|
|Journal||Angewandte Chemie - International Edition|
|Publication status||Published - 2015 Sept 1|
- activity-based probes
- monoamine oxidases
- Parkinson's disease
ASJC Scopus subject areas