A smartphone fluorescence imaging-based mobile biosensing system integrated with a passive fluidic control cartridge for minimal user intervention and high accuracy

Won Il Lee, Younghyeon Park, Jaemin Park, Sajal Shrivastava, Young Min Son, Hak Jong Choi, Jaelin Lee, Byeungwoo Jeon, Heon Lee, Nae Eung Lee

Research output: Contribution to journalArticle

Abstract

A key challenge for realizing mobile device-based on-the-spot environmental biodetection is that a biosensor integrated with a fluid handling sensor cartridge must have acceptable accuracy comparable to that of conventional standard analytical methods. Furthermore, the user interface must be easy to operate, technologically plausible, and concise. Herein, we introduced an advanced smartphone imaging-based fluorescence microscope designed for Hg 2+ monitoring by utilizing a biosensor cartridge that reduced user intervention via time-sequenced passive fluid handling. The cartridge also employed a metal-nanostructured plastic substrate for complementing the fluorescence signal output; this helped the realization of high-accuracy detection, in which a ratiometric dual-wavelength detection method was applied. Using 30 samples of Hg 2+ -spiked wastewater, we showed that our device, which has a detection limit of ∼1 pM, can perform analytical assays accurately. The detection results from our method were in good linearity and agreement with those of conventional standard methods. We conclude that the integration of a simple-to-use biosensor cartridge, fluorescence signal-enhancing substrate, dual-wavelength detection, and quantitative image data processing on a smartphone has great potential to make any population accessible to small-molecule detection, which has been performed in centralized laboratories for environmental monitoring.

Original languageEnglish
Pages (from-to)1502-1511
Number of pages10
JournalLab on a Chip
Volume19
Issue number8
DOIs
Publication statusPublished - 2019 Jan 1

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Optical Imaging
Smartphones
Fluidics
Biosensors
Biosensing Techniques
Fluorescence
Imaging techniques
Wavelength
Fluids
Monitoring
Substrates
Mobile devices
Equipment and Supplies
User interfaces
Environmental Monitoring
Assays
Wastewater
Microscopes
Waste Water
Metals

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

A smartphone fluorescence imaging-based mobile biosensing system integrated with a passive fluidic control cartridge for minimal user intervention and high accuracy. / Lee, Won Il; Park, Younghyeon; Park, Jaemin; Shrivastava, Sajal; Son, Young Min; Choi, Hak Jong; Lee, Jaelin; Jeon, Byeungwoo; Lee, Heon; Lee, Nae Eung.

In: Lab on a Chip, Vol. 19, No. 8, 01.01.2019, p. 1502-1511.

Research output: Contribution to journalArticle

Lee, Won Il ; Park, Younghyeon ; Park, Jaemin ; Shrivastava, Sajal ; Son, Young Min ; Choi, Hak Jong ; Lee, Jaelin ; Jeon, Byeungwoo ; Lee, Heon ; Lee, Nae Eung. / A smartphone fluorescence imaging-based mobile biosensing system integrated with a passive fluidic control cartridge for minimal user intervention and high accuracy. In: Lab on a Chip. 2019 ; Vol. 19, No. 8. pp. 1502-1511.
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