A strategy for sensitivity and specificity enhancements in prostate specific antigen-α1-antichymotrypsin detection based on surface plasmon resonance

Cuong Cao, Jun Pyo Kim, Byung Woo Kim, Heeyeop Chae, Hyun C. Yoon, Sang Sik Yang, Sang Jun Sim

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

A biochip based on surface plasmon resonance was fabricated to detect prostate specific antigen-α1-antichymotrypsin (PSA-ACT complex) in both HBS buffer and human serum. To reduce non-specific binding and steric hindrance effect, the chemical surface of the sensor chips was constructed by using various oligo(ethylene glycol) mixtures of different molar ratios of HS(CH2)11(OCH2CH2)6OCH2COOH and HS(CH2)11(OCH2CH2)3OH. The self-assembled monolayers were biotinylated to facilitate the immobilization of streptavidin. Using the chip surfaces, PSA-ACT complex in HBS buffer and human serum was detected at 20.7 and 47.5 ng/ml by primary immunoresponse, respectively. However, the limit of detection could be simply enhanced by a sandwich strategy to improve the sensitivity and specificity of the immunoassay. An intact PSA polyclonal antibody was used as an amplifying agent in the strategy. As a result, PSA-ACT complex concentrations as low as 10.2 and 18.1 ng/ml were found in the HBS buffer and human serum sample, respectively. The result indicates that this approach could satisfy our goal without modifying the secondary interactant.

Original languageEnglish
Pages (from-to)2106-2113
Number of pages8
JournalBiosensors and Bioelectronics
Volume21
Issue number11
DOIs
Publication statusPublished - 2006 May 15
Externally publishedYes

Fingerprint

Surface Plasmon Resonance
Surface plasmon resonance
Prostate-Specific Antigen
Antigens
Buffers
Biochips
Sensitivity and Specificity
Self assembled monolayers
Ethylene glycol
Serum
Antibodies
Streptavidin
Ethylene Glycol
Immunoassay
Immobilization
Limit of Detection
Sensors

Keywords

  • Biotinylation
  • Enhancement
  • Oligo(ethylene glycol)
  • Prostate specific antigen
  • Self-assembled monolayer
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

A strategy for sensitivity and specificity enhancements in prostate specific antigen-α1-antichymotrypsin detection based on surface plasmon resonance. / Cao, Cuong; Kim, Jun Pyo; Kim, Byung Woo; Chae, Heeyeop; Yoon, Hyun C.; Yang, Sang Sik; Sim, Sang Jun.

In: Biosensors and Bioelectronics, Vol. 21, No. 11, 15.05.2006, p. 2106-2113.

Research output: Contribution to journalArticle

Cao, Cuong ; Kim, Jun Pyo ; Kim, Byung Woo ; Chae, Heeyeop ; Yoon, Hyun C. ; Yang, Sang Sik ; Sim, Sang Jun. / A strategy for sensitivity and specificity enhancements in prostate specific antigen-α1-antichymotrypsin detection based on surface plasmon resonance. In: Biosensors and Bioelectronics. 2006 ; Vol. 21, No. 11. pp. 2106-2113.
@article{1866b122c91c43dcb876b2a69dcb2fde,
title = "A strategy for sensitivity and specificity enhancements in prostate specific antigen-α1-antichymotrypsin detection based on surface plasmon resonance",
abstract = "A biochip based on surface plasmon resonance was fabricated to detect prostate specific antigen-α1-antichymotrypsin (PSA-ACT complex) in both HBS buffer and human serum. To reduce non-specific binding and steric hindrance effect, the chemical surface of the sensor chips was constructed by using various oligo(ethylene glycol) mixtures of different molar ratios of HS(CH2)11(OCH2CH2)6OCH2COOH and HS(CH2)11(OCH2CH2)3OH. The self-assembled monolayers were biotinylated to facilitate the immobilization of streptavidin. Using the chip surfaces, PSA-ACT complex in HBS buffer and human serum was detected at 20.7 and 47.5 ng/ml by primary immunoresponse, respectively. However, the limit of detection could be simply enhanced by a sandwich strategy to improve the sensitivity and specificity of the immunoassay. An intact PSA polyclonal antibody was used as an amplifying agent in the strategy. As a result, PSA-ACT complex concentrations as low as 10.2 and 18.1 ng/ml were found in the HBS buffer and human serum sample, respectively. The result indicates that this approach could satisfy our goal without modifying the secondary interactant.",
keywords = "Biotinylation, Enhancement, Oligo(ethylene glycol), Prostate specific antigen, Self-assembled monolayer, Surface plasmon resonance",
author = "Cuong Cao and Kim, {Jun Pyo} and Kim, {Byung Woo} and Heeyeop Chae and Yoon, {Hyun C.} and Yang, {Sang Sik} and Sim, {Sang Jun}",
year = "2006",
month = "5",
day = "15",
doi = "10.1016/j.bios.2005.10.014",
language = "English",
volume = "21",
pages = "2106--2113",
journal = "Biosensors",
issn = "0956-5663",
publisher = "Elsevier Limited",
number = "11",

}

TY - JOUR

T1 - A strategy for sensitivity and specificity enhancements in prostate specific antigen-α1-antichymotrypsin detection based on surface plasmon resonance

AU - Cao, Cuong

AU - Kim, Jun Pyo

AU - Kim, Byung Woo

AU - Chae, Heeyeop

AU - Yoon, Hyun C.

AU - Yang, Sang Sik

AU - Sim, Sang Jun

PY - 2006/5/15

Y1 - 2006/5/15

N2 - A biochip based on surface plasmon resonance was fabricated to detect prostate specific antigen-α1-antichymotrypsin (PSA-ACT complex) in both HBS buffer and human serum. To reduce non-specific binding and steric hindrance effect, the chemical surface of the sensor chips was constructed by using various oligo(ethylene glycol) mixtures of different molar ratios of HS(CH2)11(OCH2CH2)6OCH2COOH and HS(CH2)11(OCH2CH2)3OH. The self-assembled monolayers were biotinylated to facilitate the immobilization of streptavidin. Using the chip surfaces, PSA-ACT complex in HBS buffer and human serum was detected at 20.7 and 47.5 ng/ml by primary immunoresponse, respectively. However, the limit of detection could be simply enhanced by a sandwich strategy to improve the sensitivity and specificity of the immunoassay. An intact PSA polyclonal antibody was used as an amplifying agent in the strategy. As a result, PSA-ACT complex concentrations as low as 10.2 and 18.1 ng/ml were found in the HBS buffer and human serum sample, respectively. The result indicates that this approach could satisfy our goal without modifying the secondary interactant.

AB - A biochip based on surface plasmon resonance was fabricated to detect prostate specific antigen-α1-antichymotrypsin (PSA-ACT complex) in both HBS buffer and human serum. To reduce non-specific binding and steric hindrance effect, the chemical surface of the sensor chips was constructed by using various oligo(ethylene glycol) mixtures of different molar ratios of HS(CH2)11(OCH2CH2)6OCH2COOH and HS(CH2)11(OCH2CH2)3OH. The self-assembled monolayers were biotinylated to facilitate the immobilization of streptavidin. Using the chip surfaces, PSA-ACT complex in HBS buffer and human serum was detected at 20.7 and 47.5 ng/ml by primary immunoresponse, respectively. However, the limit of detection could be simply enhanced by a sandwich strategy to improve the sensitivity and specificity of the immunoassay. An intact PSA polyclonal antibody was used as an amplifying agent in the strategy. As a result, PSA-ACT complex concentrations as low as 10.2 and 18.1 ng/ml were found in the HBS buffer and human serum sample, respectively. The result indicates that this approach could satisfy our goal without modifying the secondary interactant.

KW - Biotinylation

KW - Enhancement

KW - Oligo(ethylene glycol)

KW - Prostate specific antigen

KW - Self-assembled monolayer

KW - Surface plasmon resonance

UR - http://www.scopus.com/inward/record.url?scp=33645843946&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645843946&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2005.10.014

DO - 10.1016/j.bios.2005.10.014

M3 - Article

C2 - 16310353

AN - SCOPUS:33645843946

VL - 21

SP - 2106

EP - 2113

JO - Biosensors

JF - Biosensors

SN - 0956-5663

IS - 11

ER -