A stress-responsive caleosin-like protein, AtCLO4, Acts as a Negative Regulator of ABA Responses in Arabidopsis

Yun Young Kim, Kwang Wook Jung, Kyoung Shin Yoo, Ji Ung Jeung, Jeong Sheop Shin

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Caleosins or related sequences have been found in a wide range of higher plants. In Arabidopsis, seed-specific caleosins are viewed as oil-body (OB)-associated proteins that possess Ca2+-dependent peroxygenase activity and are involved in processes of lipid degradation. Recent experimental evidence suggests that one of the Arabidopsis non-seed caleosins, AtCLO3, is involved in controlling stomatal aperture during the drought response; the roles of the other caleosin-like proteins in Arabidopsis remain largely uncharacterized. We have demonstrated that a novel stress-responsive and OB-associated Ca2+-binding caleosin-like protein, AtCLO4, is expressed in non-seed tissues of Arabidopsis, including guard cells, and down-regulated following exposure to exogenous ABA and salt stress. At the seed germination stage, a loss-of-function mutant (atclo4) was hypersensitive to ABA, salt and mannitol stresses, whereas AtCLO4-overexpressing (Ox) lines were more hyposensitive to those stresses than the wild type. In adult stage, atclo4 mutant and AtCLO4-Ox plants showed enhanced and decreased drought tolerance, respectively. Following exposure to exogenous ABA, the expression of key ABA-dependent regulatory genes, such as ABF3 and ABF4, was up-regulated in the atclo4 mutant, while it was down-regulated in AtCLO4-Ox lines. Based on these results, we propose that the OB-associated Ca2+-binding AtCLO4 protein acts as a negative regulator of ABA responses in Arabidopsis.

Original languageEnglish
Pages (from-to)874-884
Number of pages11
JournalPlant and Cell Physiology
Volume52
Issue number5
DOIs
Publication statusPublished - 2011 May 1

Fingerprint

Arabidopsis
lipid bodies
Droughts
Proteins
calcium
proteins
mutants
Seeds
Salts
Arabidopsis Proteins
guard cells
Mannitol
Regulator Genes
Germination
regulator genes
mannitol
drought tolerance
salt stress
binding proteins
seed germination

Keywords

  • ABA response
  • Arabidopsis thaliana
  • CaBP
  • Caleosin
  • Drought tolerance
  • Stomatal control

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

A stress-responsive caleosin-like protein, AtCLO4, Acts as a Negative Regulator of ABA Responses in Arabidopsis. / Kim, Yun Young; Jung, Kwang Wook; Yoo, Kyoung Shin; Jeung, Ji Ung; Shin, Jeong Sheop.

In: Plant and Cell Physiology, Vol. 52, No. 5, 01.05.2011, p. 874-884.

Research output: Contribution to journalArticle

Kim, Yun Young ; Jung, Kwang Wook ; Yoo, Kyoung Shin ; Jeung, Ji Ung ; Shin, Jeong Sheop. / A stress-responsive caleosin-like protein, AtCLO4, Acts as a Negative Regulator of ABA Responses in Arabidopsis. In: Plant and Cell Physiology. 2011 ; Vol. 52, No. 5. pp. 874-884.
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