A stress-responsive Escherichia coli protein, CysQ is a highly effective solubility enhancer for aggregation-prone heterologous proteins

Jong Hwan Lee, Ji Yun Lee, Jong Am Song, Kyung Yeon Han, Doo Sung Lee, Jeewon Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

When used as an N-terminal fusion expression partner, the Escherichia coli stress-responsive protein, CysQ dramatically increased the cytoplasmic solubility of various aggregation-prone heterologous proteins: Pseudomonas putida cutinase (CUT), human granulocyte colony-stimulating factor (hG-CSF), human ferritin light chain (hFTN-L), arginine deiminase (ADI), human interleukin-2 (IL2), human activation induced cytidine deaminase (AID), and deletion mutant of human glutamate decarboxylase (GAD448-585). As compared with well-known fusion tags such as glutathione-S-transferase (GST) and maltose-binding protein (MBP), the performance of CysQ as solubility enhancer was evidently better than GST and was similar to or better than MBP for the seven heterologous proteins above. This is likely due to the intrinsic ability of CysQ to form its native conformation, probably promoting the binding of molecular chaperones during the folding of CysQ-fusion protein. When used as a substrate, p-nitrophenyl butyrate (PNB) was successfully hydrolyzed to p-nitrophenol by CysQ-CUT fusion mutant. Even after CysQ was removed, the solubility of hFTN-L and hG-CSF, the secondary structure of hG-CSF, and self-assembly activity of hFTN-L were successfully maintained. Conclusively, it seems that CysQ is a highly effective solubility enhancer and fusion expression partner for the production of a variety of bio-active recombinant proteins.

Original languageEnglish
Pages (from-to)91-98
Number of pages8
JournalProtein Expression and Purification
Volume101
DOIs
Publication statusPublished - 2014 Jan 1

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Escherichia coli Proteins
Solubility
Apoferritins
Granulocyte Colony-Stimulating Factor
Proteins
Maltose-Binding Proteins
Glutathione Transferase
Pseudomonas putida
Molecular Chaperones
Glutamate Decarboxylase
Heat-Shock Proteins
Recombinant Proteins
Human Activities
Interleukin-2

ASJC Scopus subject areas

  • Biotechnology

Cite this

A stress-responsive Escherichia coli protein, CysQ is a highly effective solubility enhancer for aggregation-prone heterologous proteins. / Lee, Jong Hwan; Lee, Ji Yun; Song, Jong Am; Han, Kyung Yeon; Lee, Doo Sung; Lee, Jeewon.

In: Protein Expression and Purification, Vol. 101, 01.01.2014, p. 91-98.

Research output: Contribution to journalArticle

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