Abstract
Aeration diffusers in wastewater treatment plants generate air bubbles that promote mixing, distribution of dissolved oxygen, and microbial processing of dissolved and suspended matter in bulk solution. Biofouling of diffusers represents a significant problem to wastewater treatment plants because biofilms decrease oxygen transfer efficiency and increase backpressure on the blower. To better understand biofouling, we conducted a pilot study to survey the bacterial community composition and function of biofilms on different diffuser substrates and compare them to those in the bulk solution. DNA was extracted from the surface of ethylene–propylene-diene monomer (EPDM), polyurethane, and silicone diffusers operated for 15 months in a municipal treatment plant and sampled at 3 and 9 months. The bacterial community composition and function of the biofilms and bulk solution were determined by amplifying the 16S rRNA genes and pyrosequencing the amplicons and raw metagenomic DNA. The ordination plots and dendrograms of the 16S rRNA and functional genes showed that while the bacterial community composition and function of the bulk solution was independent of sampling time, the composition and function of the biofilms differed by diffuser type and testing time. For the EPDM and silicone diffusers, the biofilm communities were more similar in composition to the bulk solution at 3 months than 9 months. In contrast, the bacteria on the polyurethane diffusers were more dissimilar to the bulk solution at 3 months than 9 months. Taken together, the survey showed that the community composition and function of bacterial biofilms depend on the diffuser substrate and testing time, which warrants further elucidation.
| Original language | English |
|---|---|
| Pages (from-to) | 6361-6373 |
| Number of pages | 13 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 100 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - 2016 Jul 1 |
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Keywords
- Aeration diffusers
- Biofouling
- Next-generation sequencing
- Wastewater
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
A survey of biofilms on wastewater aeration diffusers suggests bacterial community composition and function vary by substrate type and time. / Noble, Peter A.; Park, Hee-Deung; Olson, Betty H.; Asvapathanagul, Pitiporn; Hunter, M. Colby; Garrido-Baserba, Manel; Lee, Sang Hoon; Rosso, Diego.
In: Applied Microbiology and Biotechnology, Vol. 100, No. 14, 01.07.2016, p. 6361-6373.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A survey of biofilms on wastewater aeration diffusers suggests bacterial community composition and function vary by substrate type and time
AU - Noble, Peter A.
AU - Park, Hee-Deung
AU - Olson, Betty H.
AU - Asvapathanagul, Pitiporn
AU - Hunter, M. Colby
AU - Garrido-Baserba, Manel
AU - Lee, Sang Hoon
AU - Rosso, Diego
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Aeration diffusers in wastewater treatment plants generate air bubbles that promote mixing, distribution of dissolved oxygen, and microbial processing of dissolved and suspended matter in bulk solution. Biofouling of diffusers represents a significant problem to wastewater treatment plants because biofilms decrease oxygen transfer efficiency and increase backpressure on the blower. To better understand biofouling, we conducted a pilot study to survey the bacterial community composition and function of biofilms on different diffuser substrates and compare them to those in the bulk solution. DNA was extracted from the surface of ethylene–propylene-diene monomer (EPDM), polyurethane, and silicone diffusers operated for 15 months in a municipal treatment plant and sampled at 3 and 9 months. The bacterial community composition and function of the biofilms and bulk solution were determined by amplifying the 16S rRNA genes and pyrosequencing the amplicons and raw metagenomic DNA. The ordination plots and dendrograms of the 16S rRNA and functional genes showed that while the bacterial community composition and function of the bulk solution was independent of sampling time, the composition and function of the biofilms differed by diffuser type and testing time. For the EPDM and silicone diffusers, the biofilm communities were more similar in composition to the bulk solution at 3 months than 9 months. In contrast, the bacteria on the polyurethane diffusers were more dissimilar to the bulk solution at 3 months than 9 months. Taken together, the survey showed that the community composition and function of bacterial biofilms depend on the diffuser substrate and testing time, which warrants further elucidation.
AB - Aeration diffusers in wastewater treatment plants generate air bubbles that promote mixing, distribution of dissolved oxygen, and microbial processing of dissolved and suspended matter in bulk solution. Biofouling of diffusers represents a significant problem to wastewater treatment plants because biofilms decrease oxygen transfer efficiency and increase backpressure on the blower. To better understand biofouling, we conducted a pilot study to survey the bacterial community composition and function of biofilms on different diffuser substrates and compare them to those in the bulk solution. DNA was extracted from the surface of ethylene–propylene-diene monomer (EPDM), polyurethane, and silicone diffusers operated for 15 months in a municipal treatment plant and sampled at 3 and 9 months. The bacterial community composition and function of the biofilms and bulk solution were determined by amplifying the 16S rRNA genes and pyrosequencing the amplicons and raw metagenomic DNA. The ordination plots and dendrograms of the 16S rRNA and functional genes showed that while the bacterial community composition and function of the bulk solution was independent of sampling time, the composition and function of the biofilms differed by diffuser type and testing time. For the EPDM and silicone diffusers, the biofilm communities were more similar in composition to the bulk solution at 3 months than 9 months. In contrast, the bacteria on the polyurethane diffusers were more dissimilar to the bulk solution at 3 months than 9 months. Taken together, the survey showed that the community composition and function of bacterial biofilms depend on the diffuser substrate and testing time, which warrants further elucidation.
KW - Aeration diffusers
KW - Biofouling
KW - Next-generation sequencing
KW - Wastewater
UR - http://www.scopus.com/inward/record.url?scp=84974779219&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84974779219&partnerID=8YFLogxK
U2 - 10.1007/s00253-016-7604-7
DO - 10.1007/s00253-016-7604-7
M3 - Article
C2 - 27294381
AN - SCOPUS:84974779219
VL - 100
SP - 6361
EP - 6373
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 14
ER -