A survey of biofilms on wastewater aeration diffusers suggests bacterial community composition and function vary by substrate type and time

Peter A. Noble, Hee-Deung Park, Betty H. Olson, Pitiporn Asvapathanagul, M. Colby Hunter, Manel Garrido-Baserba, Sang Hoon Lee, Diego Rosso

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aeration diffusers in wastewater treatment plants generate air bubbles that promote mixing, distribution of dissolved oxygen, and microbial processing of dissolved and suspended matter in bulk solution. Biofouling of diffusers represents a significant problem to wastewater treatment plants because biofilms decrease oxygen transfer efficiency and increase backpressure on the blower. To better understand biofouling, we conducted a pilot study to survey the bacterial community composition and function of biofilms on different diffuser substrates and compare them to those in the bulk solution. DNA was extracted from the surface of ethylene–propylene-diene monomer (EPDM), polyurethane, and silicone diffusers operated for 15 months in a municipal treatment plant and sampled at 3 and 9 months. The bacterial community composition and function of the biofilms and bulk solution were determined by amplifying the 16S rRNA genes and pyrosequencing the amplicons and raw metagenomic DNA. The ordination plots and dendrograms of the 16S rRNA and functional genes showed that while the bacterial community composition and function of the bulk solution was independent of sampling time, the composition and function of the biofilms differed by diffuser type and testing time. For the EPDM and silicone diffusers, the biofilm communities were more similar in composition to the bulk solution at 3 months than 9 months. In contrast, the bacteria on the polyurethane diffusers were more dissimilar to the bulk solution at 3 months than 9 months. Taken together, the survey showed that the community composition and function of bacterial biofilms depend on the diffuser substrate and testing time, which warrants further elucidation.

Original languageEnglish
Pages (from-to)6361-6373
Number of pages13
JournalApplied Microbiology and Biotechnology
Volume100
Issue number14
DOIs
Publication statusPublished - 2016 Jul 1

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Biofilms
Waste Water
Biofouling
Polyurethanes
Silicones
rRNA Genes
Oxygen
Metagenomics
DNA
Surveys and Questionnaires
Air
Bacteria

Keywords

  • Aeration diffusers
  • Biofouling
  • Next-generation sequencing
  • Wastewater

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

A survey of biofilms on wastewater aeration diffusers suggests bacterial community composition and function vary by substrate type and time. / Noble, Peter A.; Park, Hee-Deung; Olson, Betty H.; Asvapathanagul, Pitiporn; Hunter, M. Colby; Garrido-Baserba, Manel; Lee, Sang Hoon; Rosso, Diego.

In: Applied Microbiology and Biotechnology, Vol. 100, No. 14, 01.07.2016, p. 6361-6373.

Research output: Contribution to journalArticle

Noble, Peter A. ; Park, Hee-Deung ; Olson, Betty H. ; Asvapathanagul, Pitiporn ; Hunter, M. Colby ; Garrido-Baserba, Manel ; Lee, Sang Hoon ; Rosso, Diego. / A survey of biofilms on wastewater aeration diffusers suggests bacterial community composition and function vary by substrate type and time. In: Applied Microbiology and Biotechnology. 2016 ; Vol. 100, No. 14. pp. 6361-6373.
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