@article{df2c23c4390f411d8ed5ca7bd147e237,
title = "A therapeutic neutralizing antibody targeting receptor binding domain of SARS-CoV-2 spike protein",
abstract = "Vaccines and therapeutics are urgently needed for the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we screen human monoclonal antibodies (mAb) targeting the receptor binding domain (RBD) of the viral spike protein via antibody library constructed from peripheral blood mononuclear cells of a convalescent patient. The CT-P59 mAb potently neutralizes SARS-CoV-2 isolates including the D614G variant without antibody-dependent enhancement effect. Complex crystal structure of CT-P59 Fab/RBD shows that CT-P59 blocks interaction regions of RBD for angiotensin converting enzyme 2 (ACE2) receptor with an orientation that is notably different from previously reported RBD-targeting mAbs. Furthermore, therapeutic effects of CT-P59 are evaluated in three animal models (ferret, hamster, and rhesus monkey), demonstrating a substantial reduction in viral titer along with alleviation of clinical symptoms. Therefore, CT-P59 may be a promising therapeutic candidate for COVID-19.",
author = "Cheolmin Kim and Ryu, {Dong Kyun} and Jihun Lee and Kim, {Young Il} and Seo, {Ji Min} and Kim, {Yeon Gil} and Jeong, {Jae Hee} and Minsoo Kim and Kim, {Jong In} and Pankyeom Kim and Bae, {Jin Soo} and Shim, {Eun Yeong} and Lee, {Min Seob} and Kim, {Man Su} and Hanmi Noh and Park, {Geun Soo} and Park, {Jae Sang} and Dain Son and Yongjin An and Lee, {Jeong No} and Kwon, {Ki Sung} and Lee, {Joo Yeon} and Hansaem Lee and Yang, {Jeong Sun} and Kim, {Kyung Chang} and Kim, {Sung Soon} and Woo, {Hye Min} and Kim, {Jun Won} and Park, {Man Seong} and Yu, {Kwang Min} and Kim, {Se Mi} and Kim, {Eun Ha} and Park, {Su Jin} and Jeong, {Seong Tae} and Yu, {Chi Ho} and Youngjo Song and Gu, {Se Hun} and Hanseul Oh and Koo, {Bon Sang} and Hong, {Jung Joo} and Ryu, {Choong Min} and Park, {Wan Beom} and Oh, {Myoung don} and Choi, {Young Ki} and Lee, {Soo Young}",
note = "Funding Information: This study was supported by a Korea National Institute of Health fund (2020-ER5311-00, 2020-ER5323-00, 2019-NI-077-01, 2019-NG-044-01), grants (011555-012664201) from the Agency for Defense Development, grants (PRM1752011) from the Ministry of Science and ICT, and partially supported by the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program (KGM9942011 and KGM4572013), Republic of Korea. Funding Information: Patents have been filed for CT-P59. C.K., D.K.R., J.L., J.M.S., M.K., J.I.K., P.K., J.S.B., E.Y.S., M.S.L., M.S.K., H.N., G.S.P., J.S.P., D.S., Y.A., J.N.L., K.S.K., and S.Y.L. are employees of Celltrion, Inc. This work was funded by Celltrion, Inc. and several grants listed in Acknowledgements. The remaining authors declare no competing interests. Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = dec,
doi = "10.1038/s41467-020-20602-5",
language = "English",
volume = "12",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}
