TY - GEN
T1 - A three-dimensional in vitro model of lymphangiogenesis in tumor microenvironment
AU - Cho, Youngkyu
AU - Na, Kyuhwan
AU - Won, Jihee
AU - Jun, Yesl
AU - Yang, Ji Hun
AU - Chung, Seok
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Here, we presented ECM incorporated new macro-fluidic platform for physiological relevant lymphangiogenesis model which has lumen structure and button-like junctions. Using this model, we successfully developed tumor microenvironment applying cancer spheroid which has hypoxic core. Interestingly, vascular endothelial growth factors (VEGFs) combination and induced interstitial flow in tumor site facilitate lymphangiogensis and promote cancer metastasis. Furthermore, this platform has a possibility for adaptive immune model since DC shows migration to new lymphatic vessel and transmigration through the lymphatic endothelium. Moreover, the macro-fluidic platform can be performed biological analysis on a chip, such as qRT-PCR, western blot, ELISA, immunostaining and monitoring in real time for cell development and migration.
AB - Here, we presented ECM incorporated new macro-fluidic platform for physiological relevant lymphangiogenesis model which has lumen structure and button-like junctions. Using this model, we successfully developed tumor microenvironment applying cancer spheroid which has hypoxic core. Interestingly, vascular endothelial growth factors (VEGFs) combination and induced interstitial flow in tumor site facilitate lymphangiogensis and promote cancer metastasis. Furthermore, this platform has a possibility for adaptive immune model since DC shows migration to new lymphatic vessel and transmigration through the lymphatic endothelium. Moreover, the macro-fluidic platform can be performed biological analysis on a chip, such as qRT-PCR, western blot, ELISA, immunostaining and monitoring in real time for cell development and migration.
KW - Lymphangiognesis
KW - Tumor microenvironment
UR - http://www.scopus.com/inward/record.url?scp=85079903473&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079903473&partnerID=8YFLogxK
M3 - Conference contribution
T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
SP - 245
EP - 247
BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PB - Chemical and Biological Microsystems Society
T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Y2 - 11 November 2018 through 15 November 2018
ER -
