A transient, microfluidic approach to the investigation of erythrocyte aggregation

The threshold shear-stress for erythrocyte disaggregation

Sehyun Shin, Jeong Hoon Nam, Jian Xun Hou, Jang Soo Suh

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Detailed analysis of red blood cells (RBCs) aggregation is often required in various clinical studies. Most conventional aggregation indices are dimensionless values and not available for comparison of across studies. Quite recently, we have developed microfluidic aggregometry that enables us to yield a critical shear-stress that are required to aggregate RBCs under the shearing hydrodynamic force. The present study investigated the relationships between the values of the critical shear-stress and conventional aggregation indices by comparing the critical shear-stress measured by the microfluidic aggregometry with the threshold shear-stress measured using a LORCA aggregometer. The results showed that the critical shear-stress did not vary with the hematocrit value while the threshold shear-rate decreased with the hematocrit value. The threshold shear-stress also showed the same hematocrit-independence as the critical shear-stress. These findings assist in rheologically validating the critical shear-stress, as defined in the microfluidic aggregometry, within the present range of hematocrit values.

Original languageEnglish
Pages (from-to)117-125
Number of pages9
JournalClinical Hemorheology and Microcirculation
Volume42
Issue number2
DOIs
Publication statusPublished - 2009 Jul 31

Fingerprint

Erythrocyte Aggregation
Microfluidics
Hematocrit
Erythrocytes
Cell Aggregation
Hydrodynamics

Keywords

  • Erythrocyte aggregation
  • Microfluidic
  • Shear-stress
  • Threshold

ASJC Scopus subject areas

  • Hematology
  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

A transient, microfluidic approach to the investigation of erythrocyte aggregation : The threshold shear-stress for erythrocyte disaggregation. / Shin, Sehyun; Nam, Jeong Hoon; Hou, Jian Xun; Suh, Jang Soo.

In: Clinical Hemorheology and Microcirculation, Vol. 42, No. 2, 31.07.2009, p. 117-125.

Research output: Contribution to journalArticle

@article{720b145fa0094f84a525b7c20eec9f9e,
title = "A transient, microfluidic approach to the investigation of erythrocyte aggregation: The threshold shear-stress for erythrocyte disaggregation",
abstract = "Detailed analysis of red blood cells (RBCs) aggregation is often required in various clinical studies. Most conventional aggregation indices are dimensionless values and not available for comparison of across studies. Quite recently, we have developed microfluidic aggregometry that enables us to yield a critical shear-stress that are required to aggregate RBCs under the shearing hydrodynamic force. The present study investigated the relationships between the values of the critical shear-stress and conventional aggregation indices by comparing the critical shear-stress measured by the microfluidic aggregometry with the threshold shear-stress measured using a LORCA aggregometer. The results showed that the critical shear-stress did not vary with the hematocrit value while the threshold shear-rate decreased with the hematocrit value. The threshold shear-stress also showed the same hematocrit-independence as the critical shear-stress. These findings assist in rheologically validating the critical shear-stress, as defined in the microfluidic aggregometry, within the present range of hematocrit values.",
keywords = "Erythrocyte aggregation, Microfluidic, Shear-stress, Threshold",
author = "Sehyun Shin and Nam, {Jeong Hoon} and Hou, {Jian Xun} and Suh, {Jang Soo}",
year = "2009",
month = "7",
day = "31",
doi = "10.3233/CH-2009-1191",
language = "English",
volume = "42",
pages = "117--125",
journal = "Clinical Hemorheology and Microcirculation",
issn = "1386-0291",
publisher = "IOS Press",
number = "2",

}

TY - JOUR

T1 - A transient, microfluidic approach to the investigation of erythrocyte aggregation

T2 - The threshold shear-stress for erythrocyte disaggregation

AU - Shin, Sehyun

AU - Nam, Jeong Hoon

AU - Hou, Jian Xun

AU - Suh, Jang Soo

PY - 2009/7/31

Y1 - 2009/7/31

N2 - Detailed analysis of red blood cells (RBCs) aggregation is often required in various clinical studies. Most conventional aggregation indices are dimensionless values and not available for comparison of across studies. Quite recently, we have developed microfluidic aggregometry that enables us to yield a critical shear-stress that are required to aggregate RBCs under the shearing hydrodynamic force. The present study investigated the relationships between the values of the critical shear-stress and conventional aggregation indices by comparing the critical shear-stress measured by the microfluidic aggregometry with the threshold shear-stress measured using a LORCA aggregometer. The results showed that the critical shear-stress did not vary with the hematocrit value while the threshold shear-rate decreased with the hematocrit value. The threshold shear-stress also showed the same hematocrit-independence as the critical shear-stress. These findings assist in rheologically validating the critical shear-stress, as defined in the microfluidic aggregometry, within the present range of hematocrit values.

AB - Detailed analysis of red blood cells (RBCs) aggregation is often required in various clinical studies. Most conventional aggregation indices are dimensionless values and not available for comparison of across studies. Quite recently, we have developed microfluidic aggregometry that enables us to yield a critical shear-stress that are required to aggregate RBCs under the shearing hydrodynamic force. The present study investigated the relationships between the values of the critical shear-stress and conventional aggregation indices by comparing the critical shear-stress measured by the microfluidic aggregometry with the threshold shear-stress measured using a LORCA aggregometer. The results showed that the critical shear-stress did not vary with the hematocrit value while the threshold shear-rate decreased with the hematocrit value. The threshold shear-stress also showed the same hematocrit-independence as the critical shear-stress. These findings assist in rheologically validating the critical shear-stress, as defined in the microfluidic aggregometry, within the present range of hematocrit values.

KW - Erythrocyte aggregation

KW - Microfluidic

KW - Shear-stress

KW - Threshold

UR - http://www.scopus.com/inward/record.url?scp=67649774481&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67649774481&partnerID=8YFLogxK

U2 - 10.3233/CH-2009-1191

DO - 10.3233/CH-2009-1191

M3 - Article

VL - 42

SP - 117

EP - 125

JO - Clinical Hemorheology and Microcirculation

JF - Clinical Hemorheology and Microcirculation

SN - 1386-0291

IS - 2

ER -