A two-stage minibioreactor system for continuous toxicity monitoring

Man Bock Gu, Geun Cheol Gil, Joong Hyun Kim

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

A two-stage minibioreactor system was successfully developed for continuous toxicity monitoring. This system consists of two minibioreactors in series. Recombinant Escherichia coli DPD2794 containing a RecA::luxCDABE fusion as a model strain was utilized to monitor environmental insults to DNA, with mitomycin C as a model toxicant. Pulse type exposures were used to evaluate the system's reproducibility and reliability. Step inputs of mitomycin C have been adopted to show the system's stability. The system's ability to monitor the possible upsets or accidental discharges of toxic chemicals was also evaluated with these step insults. All the data demonstrated that this two-stage minibioreactor system using recombinant bacteria containing stress promoters fused with lux genes is quite appropriate for continuous toxicity monitoring. Long-term operation and minimized media-usage have been investigated. Thus application to many different areas, including an early warning system of wastewater biotreatment plant upsets and the monitoring and tracking of accidental spills, discharges or failures in plant operation are plausible.

Original languageEnglish
Pages (from-to)355-361
Number of pages7
JournalBiosensors and Bioelectronics
Volume14
Issue number4
DOIs
Publication statusPublished - 1999 Apr 30
Externally publishedYes

Keywords

  • Bioluminescence
  • Genotoxicity
  • Recombinant Escherichia coli
  • Stress promotors
  • Two-stage minibioreactors

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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