A uterine cell mitogen distinct from epidermal growth factor in porcine uterine luminal fluids

characterization and partial purification.

R. C. Simmen, Yong Ko, X. H. Liu, M. H. Wilde, W. F. Pope, F. A. Simmen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Uterine luminal fluids (ULF) from early (Days 10 and 12)-pregnant sows contain factors that stimulate DNA synthesis in a variety of cell lines. The major growth factor component in these fluids has been partially purified 200-fold by heat treatment, anion-exchange chromatography, and gel filtration using mouse embryo-derived AKR-2B fibroblasts as an indicator cell line. The ULF mitogen (ULFM) is a polypeptide with an apparent molecular weight of 4800; it is extremely heat stable and resistant to treatment with urea. This mitogen is also present in ULF from cycling sows but is not detectable in uterine cytosolic extracts or in serum isolated from pigs at Day 12 of pregnancy. The addition of this factor to medium containing 0.5% calf serum results in a 50% increase in final cell density of AKR-2B cells. ULFM appears biologically distinct from mouse and human epidermal growth factor (EGF), since its activity is not inhibited by antibody to mouse EGF and it does not compete for binding to human (A431) EGF receptors. In addition, the ULF factor stimulates DNA synthesis in human A431 epidermoid carcinoma cells, whereas EGF is inhibitory. Partially purified ULFM also stimulates DNA synthesis in primary cultures of pig uterine stromal cells. This mitogen activity is dose-dependent and is not inhibited by antibody to mouse EGF. Thus ULFM may act in concert with other peptide growth factors in regulating uterine growth and/or differentiation.

Original languageEnglish
Pages (from-to)551-561
Number of pages11
JournalBiology of Reproduction
Volume38
Issue number3
Publication statusPublished - 1988 Apr 1
Externally publishedYes

Fingerprint

Mitogens
Epidermal Growth Factor
Swine
Fibroblast Growth Factors
Intercellular Signaling Peptides and Proteins
Hot Temperature
Cell Line
Peptides
Antibodies
Stromal Cells
Serum
Gel Chromatography
Anions
Urea
Squamous Cell Carcinoma
Embryonic Structures
Fibroblasts
Cell Count
Molecular Weight
Pregnancy

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

A uterine cell mitogen distinct from epidermal growth factor in porcine uterine luminal fluids : characterization and partial purification. / Simmen, R. C.; Ko, Yong; Liu, X. H.; Wilde, M. H.; Pope, W. F.; Simmen, F. A.

In: Biology of Reproduction, Vol. 38, No. 3, 01.04.1988, p. 551-561.

Research output: Contribution to journalArticle

Simmen, R. C. ; Ko, Yong ; Liu, X. H. ; Wilde, M. H. ; Pope, W. F. ; Simmen, F. A. / A uterine cell mitogen distinct from epidermal growth factor in porcine uterine luminal fluids : characterization and partial purification. In: Biology of Reproduction. 1988 ; Vol. 38, No. 3. pp. 551-561.
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