A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils.

Sewoon Han, Ji Jing Yan, Yoojin Shin, Jessie J. Jeon, Jihee Won, Hyo Eun Jeong, Roger D. Kamm, Young Joon Kim, Seok Chung

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Spatiotemporal analysis of the inflammatory response has been limited by the difficulties of in vivo imaging and reconstitution of inflammation in vitro. Here, we present a novel method for establishing in vivo-like inflammatory models in a microfluidic device and quantitatively measuring the three-dimensional transmigration of neutrophils during the inflammatory process. This enabled us to concurrently characterize transendothelial migration behaviors of neutrophils under the influence of various inflammatory stimuli.

Original languageEnglish
Pages (from-to)3861-3865
Number of pages5
JournalLab on a Chip
Volume12
Issue number20
DOIs
Publication statusPublished - 2012 Oct 21

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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  • Cite this

    Han, S., Yan, J. J., Shin, Y., Jeon, J. J., Won, J., Jeong, H. E., Kamm, R. D., Kim, Y. J., & Chung, S. (2012). A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils. Lab on a Chip, 12(20), 3861-3865. https://doi.org/10.1039/c2lc40445a