A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils.

Sewoon Han, Ji Jing Yan, Yoojin Shin, Jessie J. Jeon, Jihee Won, Hyo Eun Jeong, Roger D. Kamm, Young Joon Kim, Seok Chung

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Spatiotemporal analysis of the inflammatory response has been limited by the difficulties of in vivo imaging and reconstitution of inflammation in vitro. Here, we present a novel method for establishing in vivo-like inflammatory models in a microfluidic device and quantitatively measuring the three-dimensional transmigration of neutrophils during the inflammatory process. This enabled us to concurrently characterize transendothelial migration behaviors of neutrophils under the influence of various inflammatory stimuli.

Original languageEnglish
Pages (from-to)3861-3865
Number of pages5
JournalLab on a chip
Volume12
Issue number20
Publication statusPublished - 2012 Oct 21

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Transendothelial and Transepithelial Migration
Lab-On-A-Chip Devices
Microfluidics
Assays
Neutrophils
Spatio-Temporal Analysis
Imaging techniques
Monitoring
Inflammation
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Han, S., Yan, J. J., Shin, Y., Jeon, J. J., Won, J., Jeong, H. E., ... Chung, S. (2012). A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils. Lab on a chip, 12(20), 3861-3865.

A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils. / Han, Sewoon; Yan, Ji Jing; Shin, Yoojin; Jeon, Jessie J.; Won, Jihee; Jeong, Hyo Eun; Kamm, Roger D.; Kim, Young Joon; Chung, Seok.

In: Lab on a chip, Vol. 12, No. 20, 21.10.2012, p. 3861-3865.

Research output: Contribution to journalArticle

Han, S, Yan, JJ, Shin, Y, Jeon, JJ, Won, J, Jeong, HE, Kamm, RD, Kim, YJ & Chung, S 2012, 'A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils.', Lab on a chip, vol. 12, no. 20, pp. 3861-3865.
Han S, Yan JJ, Shin Y, Jeon JJ, Won J, Jeong HE et al. A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils. Lab on a chip. 2012 Oct 21;12(20):3861-3865.
Han, Sewoon ; Yan, Ji Jing ; Shin, Yoojin ; Jeon, Jessie J. ; Won, Jihee ; Jeong, Hyo Eun ; Kamm, Roger D. ; Kim, Young Joon ; Chung, Seok. / A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils. In: Lab on a chip. 2012 ; Vol. 12, No. 20. pp. 3861-3865.
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