A whole cell bioluminescent biosensor for the detection of membrane-damaging toxicity

Sue Hyung Choi, Man Bock Gu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The recombinant bacteria strain DPD2540, containing a fabA::luxCDABE fusion, was used to detect the toxicity of various chemicals in this study. Membrane damaging agents such as phenol, ethanol, and cerulenin induced a rapid bioluminescent response from this strain. Other toxic agents, such as DNA-damaging or oxidative-damaging chemicals, showed a delayed bioluminescent response in which the maximum peak appeared over 150mim after induction. This strain was also tested for measurement of toxicity in field samples such as wastewater and river water effluents.

Original languageEnglish
Pages (from-to)59-62
Number of pages4
JournalBiotechnology and Bioprocess Engineering
Volume4
Issue number1
DOIs
Publication statusPublished - 1999 Jan 1

Keywords

  • Bioluminescent bacteria
  • Bioluminescent response
  • Membrane damage
  • fabA promoter
  • lux gene

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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