AAM-B interacts with nonstructural 4B and regulates hepatitis C virus propagation

Eun Mee Park, Yun Sook Lim, Byung-Yoon Ahn, Soon B. Hwang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) usurps host cellular lipid metabolism for production of infectious virus particles. Recently, we have screened a siRNA library targeting host factors that control lipid metabolism and lipid droplet (LD) formation in cell culture grown HCV (HCVcc)- infected cells. Of 10 final candidates, we selected the gene encoding AAM-B for further characterization. We showed that siRNA-mediated knockdown of AAM-B impaired HCV propagation in Jc1-infected cells. More precisely, knockdown of AAM-B abrogated production of infectious HCV particles in both Jc1 RNA electroporated cells and Jc1-infected cells. It is worth noting that knockdown of AAM-B exerted no effect on lipid droplet formation. Moreover, AAM-B interacted with nonstructural 4B (NS4B) through the C-terminal region of NS4B. Protein interplay between AAM-B and NS4B was verified in the context of HCV replication. Using either transient or stable expression of AAM-B, we verified that AAM-B colocalized with NS4B in the cytoplasm. Immunofluorescence data further showed that AAM-B might be involved in recruitment of NS4B to sites in close proximity to LDs to facilitate HCV propagation. Collectively, this study provides new insight into how HCV utilizes cellular AAM-B to facilitate viral propagation.

Original languageEnglish
Article number132839
JournalPLoS One
Volume10
Issue number7
DOIs
Publication statusPublished - 2015 Jul 17

Fingerprint

Hepatitis C virus
Viruses
Hepacivirus
small interfering RNA
virion
Lipid Metabolism
lipid metabolism
Virion
Small Interfering RNA
droplets
Hepatovirus
cells
hepatitis A
Lipids
Gene encoding
lipids
Virus Replication
virus replication
fluorescent antibody technique
Fluorescent Antibody Technique

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

AAM-B interacts with nonstructural 4B and regulates hepatitis C virus propagation. / Park, Eun Mee; Lim, Yun Sook; Ahn, Byung-Yoon; Hwang, Soon B.

In: PLoS One, Vol. 10, No. 7, 132839, 17.07.2015.

Research output: Contribution to journalArticle

Park, Eun Mee ; Lim, Yun Sook ; Ahn, Byung-Yoon ; Hwang, Soon B. / AAM-B interacts with nonstructural 4B and regulates hepatitis C virus propagation. In: PLoS One. 2015 ; Vol. 10, No. 7.
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