Aberrant promoter CpG island methylation of the hDAB2IP gene in colorectal cancer

Ji Wook Moon, Jung Ok Lee, Soo Kyung Lee, Ji Hae Kim, Ga Young You, Jin Kim, Hyeon Soo Kim, Sun Hwa Park

Research output: Contribution to journalArticle

Abstract

Human DAB2IP (hDAB2IP), a novel GTPase-activating protein modulating Ras-mediated signaling, is a candidate tumor suppressor gene. However, promoter methylation of hDAB2IP in colorectal cancer (CRC) is poorly understood. To ascertain the mechanism of gene promoter hypermethylation in CRC cells, we investigated the promoter methylation status of hDAB2IP genes in four CRC cell lines using a methylation specific polymerase chain reaction (MS-PCR). The frequency of DNA methylation was detected in three of the four CRC cell lines. The hDAB2IP gene expression was examined and determined using a reverse transcription-PCR (RT-PCR) and immunoblot analysis in CRC cell lines. Gene expression was silenced or down-regulated in SW-480 with methylated hDAB2IP, but could be restored by treatment with 5-aza-2?-deoxycytidine, a DNA methyl-transferase inhibitor. The frequency of DNA methylation was detected in 10 of 25 colon tumors (40%), compared with 0 of 25 (0%) corresponding adjacent normal colon tissues (pB0.005). These results suggest that hDAB2IP methylation is frequently present in colon cancer, and gene silencing may play an important role in colorectal carcinogenesis.

Original languageEnglish
JournalEuropean journal of Clinical and Medical Oncology
Volume3
Issue number1
Publication statusPublished - 2011 May 23

Keywords

  • Colon cancer
  • Hypermethylation
  • Methylation-specific polymerase chain reaction
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Oncology

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