Aberrant promoter CpG islands methylation of tumor suppressor genes in cholangiocarcinoma

Kyung Ok Uhm, Eun Soo Lee, Yun Mi Lee, Hyeon Soo Kim, Young Nyun Park, Sun Hwa Park

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Aberrant DNA methylation of 5′-CpG islands located within gene promoters has been identified as a mechanism for transcriptional inactivation of tumor suppressor genes. To ascertain the mechanism of gene promoter hypermethylation in cholangiocarcinoma (CC), we investigated promoter methylation status of the candidate genes ID4, DLC-1, and SFRP1 in 41 CCs, 19 adjacent nontumor tissues, and 15 normal liver tissues using methylation-specific PCR (MSP). The frequencies of DNA methylation were: 57.5% (23 of 40) for ID4, 14.3% (5 of 35) for DLC-1, and 63.4% (26 of 41) for SFRP1, respectively. In contrast, a low frequency of methylation was detected in nontumor tissues. In addition, hypermethylated status of these genes was detected in three kinds of CC cell lines. Moreover, the downregulated expression of these genes in these cells was restored by treatment with 5-aza-2′-deoxycytidine, a DNA methyltransferase inhibitor. Taken together, these results suggest that aberrant DNA methylation may contribute to the tumorigenesis of cholangiocarcinoma.

Original languageEnglish
Pages (from-to)151-157
Number of pages7
JournalOncology Research
Volume17
Issue number4
DOIs
Publication statusPublished - 2008

Keywords

  • Cholangiocarcinoma
  • Hypermethylation
  • Methylation-specific PCR
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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