Ablation of Stabilin-1 Enhances Bone-Resorbing Activity in Osteoclasts In Vitro

Soon Young Kim, Eun Hye Lee, Seung Yoon Park, Hyuck Choi, Jeong Tae Koh, Eui Kyun Park, In-San Kim, Jung Eun Kim

Research output: Contribution to journalArticle

Abstract

Stabilin-1 is a transmembrane receptor that regulates molecule recycling and cell homeostasis by controlling the intracellular trafficking and participates in cell–cell adhesion and transmigration. Stabilin-1 expression is observed in various organs, including bones; however, its function and regulatory mechanisms in the bone remain unclear. In this study, we evaluated the physiological function of stabilin-1 in bone cells and tissue using a stabilin-1 knockout (Stab1 KO) mouse model. In wild-type (WT) mice, stabilin-1 was expressed in osteoblasts and osteoclasts, and its expression was maintained during osteoblast differentiation but significantly decreased after osteoclast differentiation. There was no difference in osteoblast differentiation and function, or the expression of osteoblast differentiation markers between mesenchymal stem cells isolated from Stab1 KO and WT mice. However, osteoclast differentiation marker levels demonstrated a non-significant increase and bone-resorbing activity was significantly increased in vitro in RANKL-induced osteoclasts from Stab1-deficient bone marrow macrophages (BMMs) compared with those of WT BMMs. Microcomputed tomography showed a negligible difference between WT and Stab1 KO mice in bone volume and trabecular thickness and number. Moreover, no in vivo functional defect in bone formation by osteoblasts was observed in the Stab1 KO mice. The osteoclast surface and number showed an increased tendency in Stab1 KO mice compared to WT mice in vivo, but this difference was not statistically significant. Overall, these results indicate that Stab1 does not play an essential role in in vivo bone development and bone cell function, but it does affect in vitro osteoclast maturation and function for bone resorption.

Original languageEnglish
JournalCalcified Tissue International
DOIs
Publication statusPublished - 2019 Jan 1

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Osteoclasts
Osteoblasts
Knockout Mice
Bone and Bones
Differentiation Antigens
Bone Marrow
Macrophages
X-Ray Microtomography
Bone Development
Recycling
Bone Resorption
Mesenchymal Stromal Cells
Osteogenesis
In Vitro Techniques
bone resorption factor
Homeostasis

Keywords

  • Bone marrow macrophages
  • Bone resorption
  • Deficiency
  • Osteoclast maturation
  • Stabilin-1 (Stab1)

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

Cite this

Ablation of Stabilin-1 Enhances Bone-Resorbing Activity in Osteoclasts In Vitro. / Kim, Soon Young; Lee, Eun Hye; Park, Seung Yoon; Choi, Hyuck; Koh, Jeong Tae; Park, Eui Kyun; Kim, In-San; Kim, Jung Eun.

In: Calcified Tissue International, 01.01.2019.

Research output: Contribution to journalArticle

Kim, Soon Young ; Lee, Eun Hye ; Park, Seung Yoon ; Choi, Hyuck ; Koh, Jeong Tae ; Park, Eui Kyun ; Kim, In-San ; Kim, Jung Eun. / Ablation of Stabilin-1 Enhances Bone-Resorbing Activity in Osteoclasts In Vitro. In: Calcified Tissue International. 2019.
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