Abnormal cell division caused by inclusion bodies in E. coli; increased resistance against external stress

Kwang Kook Lee, Cheol Seong Jang, Ju Yeon Yoon, Se Yoon Kim, Tae Ho Kim, Ki Hyun Ryu, Wook Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Inclusion body formation occurs naturally in prokaryotic cells, but is particularly common when heterologous foreign proteins are overexpressed in bacterial systems. The plant disease virus protein CMV 3a (cucumber mosaic virus movement protein) and the 56 kDa Orientia tsutsugamushi (OT56) protein (an outer membrane protein), which causes tsutsugamushi disease, were expressed in Escherichia coli, and found to form inclusion bodies. Confocal laser scanning microscopy revealed that these inclusion bodies are localized at the cellular poles within E. coli. Cells expressing inclusion bodies appeared to be interconnected, and divided abnormally. The clustered cells exhibited biofilm-like characteristics in that the interior cells of the community were protected by the antibiotic resistance of the outer cells. We compared the number of colony-forming units in inclusion body-forming versus non-forming E. coli to demonstrate the effects of lysozyme, sonication or antibiotic treatment. E. coli clustering provided significantly improved protection against cell disruption/lysis by physical and biochemical stress. This is the first report that shows that abnormal cell division caused by inclusion body formation can cause cellular clustering, resulting in improved resistance to stress in vitro.

Original languageEnglish
Pages (from-to)394-402
Number of pages9
JournalMicrobiological Research
Volume163
Issue number4
DOIs
Publication statusPublished - 2008 Jul 15

Fingerprint

Inclusion Bodies
inclusion bodies
Cell Division
cell division
Escherichia coli
cells
Cluster Analysis
Proteins
Orientia tsutsugamushi
proteins
Cucumovirus
Scrub Typhus
Prokaryotic Cells
Plant Diseases
Plant Viruses
Sonication
Cytoprotection
Cucumber mosaic virus
outer membrane proteins
plant viruses

Keywords

  • Confocal laser scanning
  • Green fluorescent protein (GFP)
  • Inclusion body

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Abnormal cell division caused by inclusion bodies in E. coli; increased resistance against external stress. / Lee, Kwang Kook; Jang, Cheol Seong; Yoon, Ju Yeon; Kim, Se Yoon; Kim, Tae Ho; Ryu, Ki Hyun; Kim, Wook.

In: Microbiological Research, Vol. 163, No. 4, 15.07.2008, p. 394-402.

Research output: Contribution to journalArticle

Lee, Kwang Kook ; Jang, Cheol Seong ; Yoon, Ju Yeon ; Kim, Se Yoon ; Kim, Tae Ho ; Ryu, Ki Hyun ; Kim, Wook. / Abnormal cell division caused by inclusion bodies in E. coli; increased resistance against external stress. In: Microbiological Research. 2008 ; Vol. 163, No. 4. pp. 394-402.
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