Absolute CD4+ cell count using a plastic microchip and a microscopic cell counter

Sook Young Bae, Hae Chul Park, Jun Seo Oh, Soo Young Yoon, Dae Won Park, In Keun Choi, Hae Joong Kim, Jong Hyun Oh, Dae Sung Hur, Chanil Chung, Jun Keun Chang, J. Paul Robinson, Chae Seung Lim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have designed and evaluated the performance of a simple, rapid, and affordable method for counting CD4+ T-cells with the use of plastic microchips. This new system is an adaptation of a "nolyse, no-wash," volumetric single platform assay, and absolute CD4+ counts are determined with the use of a microscopic scanning cell counter. To assess the CD4+ count test precision and linearity of the system, measured CD4+ counts were compared with two other reference assays (single and dual platform flow cytometry) with the use of 123 clinical samples including samples obtained from 35 HIV-infected patients, and artificially diluted samples. A correlation between the results from the use of the new method and from the use of the two other reference assays was r = 0.98 for the clinical samples. A dilution test of the new method demonstrated a linearity of r ≥ 0.99, with coefficients of variation ≤7.6% for all concentration levels. Our findings suggest that the new CD4+ counting device can be potentially be applied for other diagnostic procedures that measure quantities of characteristic antigens or other materials on cells.

Original languageEnglish
Pages (from-to)345-353
Number of pages9
JournalCytometry Part B - Clinical Cytometry
Volume76
Issue number5
DOIs
Publication statusPublished - 2009

Keywords

  • Absolute CD4 count
  • Antigens
  • CD4
  • CD4 lymphocyte count
  • Flow cytometry
  • Microchip
  • Microscopic cell counter

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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