Absolute CD4+ cell count using a plastic microchip and a microscopic cell counter

Sook Young Bae, Hae Chul Park, Jun Seo Oh, Soo-Young Yoon, Dae Won Park, In Keun Choi, Hai Joong Kim, Jong Hyun Oh, Dae Sung Hur, Chanil Chung, Jun Keun Chang, J. Paul Robinson, Chae Seung Lim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have designed and evaluated the performance of a simple, rapid, and affordable method for counting CD4+ T-cells with the use of plastic microchips. This new system is an adaptation of a "nolyse, no-wash," volumetric single platform assay, and absolute CD4+ counts are determined with the use of a microscopic scanning cell counter. To assess the CD4+ count test precision and linearity of the system, measured CD4+ counts were compared with two other reference assays (single and dual platform flow cytometry) with the use of 123 clinical samples including samples obtained from 35 HIV-infected patients, and artificially diluted samples. A correlation between the results from the use of the new method and from the use of the two other reference assays was r = 0.98 for the clinical samples. A dilution test of the new method demonstrated a linearity of r ≥ 0.99, with coefficients of variation ≤7.6% for all concentration levels. Our findings suggest that the new CD4+ counting device can be potentially be applied for other diagnostic procedures that measure quantities of characteristic antigens or other materials on cells.

Original languageEnglish
Pages (from-to)345-353
Number of pages9
JournalCytometry Part B - Clinical Cytometry
Volume76
Issue number5
DOIs
Publication statusPublished - 2009 Dec 1

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Keywords

  • Absolute CD4 count
  • Antigens
  • CD4
  • CD4 lymphocyte count
  • Flow cytometry
  • Microchip
  • Microscopic cell counter

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Pathology and Forensic Medicine

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