Accelerated osteogenic differentiation of MC3T3-E1 cells by lactoferrin-conjugated nanodiamonds through enhanced anti-oxidant and anti- inflammatory effects

Sung Eun Kim, Somang Choi, Jae Young Hong, Kyu Sik Shim, Tae Hoon Kim, Kyeongsoon Park, Suk Ha Lee

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    The purpose of this study was to investigate the effects of lactoferrin (LF)-conjugated nanodiamonds (NDs) in vitro on both anti-oxidant and anti-inflammation activity as well as osteogenic promotion. The application of LF-NDs resulted in sustained release of LF for up to 7 days. In vitro anti-oxidant analyses performed using Dichlorofluorescin diacetate (DCF-DA) assay and cell proliferation studies showed that LF (50 μg)-NDs effectively scavenged the reactive oxygen species (ROS) in MC3T3-E1 cells (osteoblast-like cells) after H2O2 treatment and increased proliferation of cells after H2O2 treatment. Treatment of lipopolysaccharide (LPS)-induced MC3T3- E1 cells with LF-NDs suppressed levels of pro-inflammatory cytokines, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). In addition, LF-NDs were associated with outstanding enhancement of osteogenic activity of MC3T3-E1 cells due to increased alkaline phosphatase (ALP) and calcium deposition. Our findings suggest that LF-NDs are an important substrate for alleviating ROS effects and inflammation, as well as promoting osteogenic differentiation of cells.

    Original languageEnglish
    Article number50
    JournalNanomaterials
    Volume10
    Issue number1
    DOIs
    Publication statusPublished - 2020 Jan

    Keywords

    • Anti-inflammatory
    • Anti-oxidant
    • Lactoferrin
    • Nanodiamonds
    • Osteogenic differentiation

    ASJC Scopus subject areas

    • Chemical Engineering(all)
    • Materials Science(all)

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