Accelerated osteogenic differentiation of MC3T3-E1 cells by lactoferrin-conjugated nanodiamonds through enhanced anti-oxidant and anti- inflammatory effects

Sung Eun Kim, Somang Choi, Jae Young Hong, Kyu Sik Shim, Tae Hoon Kim, Kyeongsoon Park, Suk Ha Lee

Research output: Contribution to journalArticle


The purpose of this study was to investigate the effects of lactoferrin (LF)-conjugated nanodiamonds (NDs) in vitro on both anti-oxidant and anti-inflammation activity as well as osteogenic promotion. The application of LF-NDs resulted in sustained release of LF for up to 7 days. In vitro anti-oxidant analyses performed using Dichlorofluorescin diacetate (DCF-DA) assay and cell proliferation studies showed that LF (50 μg)-NDs effectively scavenged the reactive oxygen species (ROS) in MC3T3-E1 cells (osteoblast-like cells) after H2O2 treatment and increased proliferation of cells after H2O2 treatment. Treatment of lipopolysaccharide (LPS)-induced MC3T3- E1 cells with LF-NDs suppressed levels of pro-inflammatory cytokines, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). In addition, LF-NDs were associated with outstanding enhancement of osteogenic activity of MC3T3-E1 cells due to increased alkaline phosphatase (ALP) and calcium deposition. Our findings suggest that LF-NDs are an important substrate for alleviating ROS effects and inflammation, as well as promoting osteogenic differentiation of cells.

Original languageEnglish
Article number50
Issue number1
Publication statusPublished - 2020 Jan



  • Anti-inflammatory
  • Anti-oxidant
  • Lactoferrin
  • Nanodiamonds
  • Osteogenic differentiation

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Materials Science(all)

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