Accumulation of β-1,3-glucanase and chitinase isoforms, and salicylic acid in the DL-β-amino-n-butyric acid-induced resistance response of pepper stems to Phytophthora capsici

Byung Kook Hwang, Ji Yoon Sunwoo, Young Jin Kim, Beom Seok Kim

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74 Citations (Scopus)

Abstract

Pepper (Capsicum annuum L.) plants sprayed with DL-β-amino-n-butyric acid (BABA) were protected against Phytophthora capsici infection. BABA treatment induced the synthesis and accumulation of β-1,3-glucanases and chitinases in the stem tissues of pepper plants. Their accumulation was very pronounced in the stems challenge-inoculated with P. capsici after BABA treatment. Several β-1,3-glucanase and chitinase isoforms accumulated in BABA treated P. capsici. When analysed by immunoblot of the denatured proteins, the 20 kDa β-1,3-glucanase and 32 kDa chitinase were found in pepper stems treated with BABA and/or infected by P. capsici. BABA treatment did not stimulate capsidiol production in pepper sterns, but prior treatment led to high accumulation in P. capsici-infected ones. Unlike capsidiol production, BABA treatment triggered a dramatic increase in the endogenous levels of salicylic acid (SA) in pepper stems. The increase in endogenous SA was much pronounced in P. capsici infected stems after BABA treatment. In conclusion, the induction of resistance to P. capsici in pepper plants by BABA treatment positively correlated with the accumulation of certain β-1,3-glucanase and chitinase isoforms, and SA. These results suggest strongly that SA may act as an endogenous signal responsible for activating particular components of resistance to P. capsici and the induction of pathogenesis-related proteins such as β-1,3-glucanase and chitinase.

Original languageEnglish
Pages (from-to)305-322
Number of pages18
JournalPhysiological and Molecular Plant Pathology
Volume51
Issue number5
DOIs
Publication statusPublished - 1997 Nov 1

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Phytophthora
Phytophthora capsici
Chitinases
Butyric Acid
Salicylic Acid
induced resistance
butyric acid
chitinase
salicylic acid
pepper
Protein Isoforms
stems
capsidiol
Capsicum
Proteins
pathogenesis-related proteins
Capsicum annuum
Infection
synthesis

ASJC Scopus subject areas

  • Plant Science

Cite this

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title = "Accumulation of β-1,3-glucanase and chitinase isoforms, and salicylic acid in the DL-β-amino-n-butyric acid-induced resistance response of pepper stems to Phytophthora capsici",
abstract = "Pepper (Capsicum annuum L.) plants sprayed with DL-β-amino-n-butyric acid (BABA) were protected against Phytophthora capsici infection. BABA treatment induced the synthesis and accumulation of β-1,3-glucanases and chitinases in the stem tissues of pepper plants. Their accumulation was very pronounced in the stems challenge-inoculated with P. capsici after BABA treatment. Several β-1,3-glucanase and chitinase isoforms accumulated in BABA treated P. capsici. When analysed by immunoblot of the denatured proteins, the 20 kDa β-1,3-glucanase and 32 kDa chitinase were found in pepper stems treated with BABA and/or infected by P. capsici. BABA treatment did not stimulate capsidiol production in pepper sterns, but prior treatment led to high accumulation in P. capsici-infected ones. Unlike capsidiol production, BABA treatment triggered a dramatic increase in the endogenous levels of salicylic acid (SA) in pepper stems. The increase in endogenous SA was much pronounced in P. capsici infected stems after BABA treatment. In conclusion, the induction of resistance to P. capsici in pepper plants by BABA treatment positively correlated with the accumulation of certain β-1,3-glucanase and chitinase isoforms, and SA. These results suggest strongly that SA may act as an endogenous signal responsible for activating particular components of resistance to P. capsici and the induction of pathogenesis-related proteins such as β-1,3-glucanase and chitinase.",
author = "Hwang, {Byung Kook} and Sunwoo, {Ji Yoon} and Kim, {Young Jin} and Kim, {Beom Seok}",
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T1 - Accumulation of β-1,3-glucanase and chitinase isoforms, and salicylic acid in the DL-β-amino-n-butyric acid-induced resistance response of pepper stems to Phytophthora capsici

AU - Hwang, Byung Kook

AU - Sunwoo, Ji Yoon

AU - Kim, Young Jin

AU - Kim, Beom Seok

PY - 1997/11/1

Y1 - 1997/11/1

N2 - Pepper (Capsicum annuum L.) plants sprayed with DL-β-amino-n-butyric acid (BABA) were protected against Phytophthora capsici infection. BABA treatment induced the synthesis and accumulation of β-1,3-glucanases and chitinases in the stem tissues of pepper plants. Their accumulation was very pronounced in the stems challenge-inoculated with P. capsici after BABA treatment. Several β-1,3-glucanase and chitinase isoforms accumulated in BABA treated P. capsici. When analysed by immunoblot of the denatured proteins, the 20 kDa β-1,3-glucanase and 32 kDa chitinase were found in pepper stems treated with BABA and/or infected by P. capsici. BABA treatment did not stimulate capsidiol production in pepper sterns, but prior treatment led to high accumulation in P. capsici-infected ones. Unlike capsidiol production, BABA treatment triggered a dramatic increase in the endogenous levels of salicylic acid (SA) in pepper stems. The increase in endogenous SA was much pronounced in P. capsici infected stems after BABA treatment. In conclusion, the induction of resistance to P. capsici in pepper plants by BABA treatment positively correlated with the accumulation of certain β-1,3-glucanase and chitinase isoforms, and SA. These results suggest strongly that SA may act as an endogenous signal responsible for activating particular components of resistance to P. capsici and the induction of pathogenesis-related proteins such as β-1,3-glucanase and chitinase.

AB - Pepper (Capsicum annuum L.) plants sprayed with DL-β-amino-n-butyric acid (BABA) were protected against Phytophthora capsici infection. BABA treatment induced the synthesis and accumulation of β-1,3-glucanases and chitinases in the stem tissues of pepper plants. Their accumulation was very pronounced in the stems challenge-inoculated with P. capsici after BABA treatment. Several β-1,3-glucanase and chitinase isoforms accumulated in BABA treated P. capsici. When analysed by immunoblot of the denatured proteins, the 20 kDa β-1,3-glucanase and 32 kDa chitinase were found in pepper stems treated with BABA and/or infected by P. capsici. BABA treatment did not stimulate capsidiol production in pepper sterns, but prior treatment led to high accumulation in P. capsici-infected ones. Unlike capsidiol production, BABA treatment triggered a dramatic increase in the endogenous levels of salicylic acid (SA) in pepper stems. The increase in endogenous SA was much pronounced in P. capsici infected stems after BABA treatment. In conclusion, the induction of resistance to P. capsici in pepper plants by BABA treatment positively correlated with the accumulation of certain β-1,3-glucanase and chitinase isoforms, and SA. These results suggest strongly that SA may act as an endogenous signal responsible for activating particular components of resistance to P. capsici and the induction of pathogenesis-related proteins such as β-1,3-glucanase and chitinase.

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