Abstract
Pepper (Capsicum annuum L.) plants sprayed with DL-β-amino-n-butyric acid (BABA) were protected against Phytophthora capsici infection. BABA treatment induced the synthesis and accumulation of β-1,3-glucanases and chitinases in the stem tissues of pepper plants. Their accumulation was very pronounced in the stems challenge-inoculated with P. capsici after BABA treatment. Several β-1,3-glucanase and chitinase isoforms accumulated in BABA treated P. capsici. When analysed by immunoblot of the denatured proteins, the 20 kDa β-1,3-glucanase and 32 kDa chitinase were found in pepper stems treated with BABA and/or infected by P. capsici. BABA treatment did not stimulate capsidiol production in pepper sterns, but prior treatment led to high accumulation in P. capsici-infected ones. Unlike capsidiol production, BABA treatment triggered a dramatic increase in the endogenous levels of salicylic acid (SA) in pepper stems. The increase in endogenous SA was much pronounced in P. capsici infected stems after BABA treatment. In conclusion, the induction of resistance to P. capsici in pepper plants by BABA treatment positively correlated with the accumulation of certain β-1,3-glucanase and chitinase isoforms, and SA. These results suggest strongly that SA may act as an endogenous signal responsible for activating particular components of resistance to P. capsici and the induction of pathogenesis-related proteins such as β-1,3-glucanase and chitinase.
| Original language | English |
|---|---|
| Pages (from-to) | 305-322 |
| Number of pages | 18 |
| Journal | Physiological and Molecular Plant Pathology |
| Volume | 51 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1997 Nov 1 |
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ASJC Scopus subject areas
- Plant Science
Cite this
Accumulation of β-1,3-glucanase and chitinase isoforms, and salicylic acid in the DL-β-amino-n-butyric acid-induced resistance response of pepper stems to Phytophthora capsici. / Hwang, Byung Kook; Sunwoo, Ji Yoon; Kim, Young Jin; Kim, Beom Seok.
In: Physiological and Molecular Plant Pathology, Vol. 51, No. 5, 01.11.1997, p. 305-322.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Accumulation of β-1,3-glucanase and chitinase isoforms, and salicylic acid in the DL-β-amino-n-butyric acid-induced resistance response of pepper stems to Phytophthora capsici
AU - Hwang, Byung Kook
AU - Sunwoo, Ji Yoon
AU - Kim, Young Jin
AU - Kim, Beom Seok
PY - 1997/11/1
Y1 - 1997/11/1
N2 - Pepper (Capsicum annuum L.) plants sprayed with DL-β-amino-n-butyric acid (BABA) were protected against Phytophthora capsici infection. BABA treatment induced the synthesis and accumulation of β-1,3-glucanases and chitinases in the stem tissues of pepper plants. Their accumulation was very pronounced in the stems challenge-inoculated with P. capsici after BABA treatment. Several β-1,3-glucanase and chitinase isoforms accumulated in BABA treated P. capsici. When analysed by immunoblot of the denatured proteins, the 20 kDa β-1,3-glucanase and 32 kDa chitinase were found in pepper stems treated with BABA and/or infected by P. capsici. BABA treatment did not stimulate capsidiol production in pepper sterns, but prior treatment led to high accumulation in P. capsici-infected ones. Unlike capsidiol production, BABA treatment triggered a dramatic increase in the endogenous levels of salicylic acid (SA) in pepper stems. The increase in endogenous SA was much pronounced in P. capsici infected stems after BABA treatment. In conclusion, the induction of resistance to P. capsici in pepper plants by BABA treatment positively correlated with the accumulation of certain β-1,3-glucanase and chitinase isoforms, and SA. These results suggest strongly that SA may act as an endogenous signal responsible for activating particular components of resistance to P. capsici and the induction of pathogenesis-related proteins such as β-1,3-glucanase and chitinase.
AB - Pepper (Capsicum annuum L.) plants sprayed with DL-β-amino-n-butyric acid (BABA) were protected against Phytophthora capsici infection. BABA treatment induced the synthesis and accumulation of β-1,3-glucanases and chitinases in the stem tissues of pepper plants. Their accumulation was very pronounced in the stems challenge-inoculated with P. capsici after BABA treatment. Several β-1,3-glucanase and chitinase isoforms accumulated in BABA treated P. capsici. When analysed by immunoblot of the denatured proteins, the 20 kDa β-1,3-glucanase and 32 kDa chitinase were found in pepper stems treated with BABA and/or infected by P. capsici. BABA treatment did not stimulate capsidiol production in pepper sterns, but prior treatment led to high accumulation in P. capsici-infected ones. Unlike capsidiol production, BABA treatment triggered a dramatic increase in the endogenous levels of salicylic acid (SA) in pepper stems. The increase in endogenous SA was much pronounced in P. capsici infected stems after BABA treatment. In conclusion, the induction of resistance to P. capsici in pepper plants by BABA treatment positively correlated with the accumulation of certain β-1,3-glucanase and chitinase isoforms, and SA. These results suggest strongly that SA may act as an endogenous signal responsible for activating particular components of resistance to P. capsici and the induction of pathogenesis-related proteins such as β-1,3-glucanase and chitinase.
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U2 - 10.1006/pmpp.1997.0119
DO - 10.1006/pmpp.1997.0119
M3 - Article
AN - SCOPUS:0031447885
VL - 51
SP - 305
EP - 322
JO - Physiological and Molecular Plant Pathology
JF - Physiological and Molecular Plant Pathology
SN - 0885-5765
IS - 5
ER -