ACE activity post-race is influenced by furosemide administration

M. F. De Mello Costa, F. A. Ronchi, Yoonsuh Jung, A. Ivanow, J. V. Braga, M. T. Ramos, D. E. Casarini, R. F. Slocombe

Research output: Contribution to journalArticle

Abstract

Exercise induced pulmonary haemorrhage (EIPH) affecting racehorses continues to raise questions regarding animal welfare and to-date no effective treatment has been identified. The mode of action of furosemide on EIPH, the only medication for the condition accepted in some racing jurisdictions, has not been completely elucidated. This research investigated the interaction between furosemide on angiotensin converting enzyme (ACE) as a potential pathway for future investigation of EIPH treatment options in a prospective case-control analytical study. ACE is a potent vasoconstrictor and substances reducing its activity could potentially contribute to decreasing blood pressure and EIPH. Horses racing on 8 official race days at Gávea Racetrack, Brazil had respiratory endoscopy data and blood samples collected after the race and were grouped into furosemide medicated and non-furosemide medicated horses. ACE measurement was conducted using fluorescence in a previously validated method. Environmental, race and haematological data were also recorded. A multiple regression model was used to analyse the data collected, with further analysis including Fisher's exact test and Pearson's chi-squared test with Yates' continuity correction; a Welch two sample t-test and a simple linear regression model. 73 horses were included in the study. ACE activity between horses not medicated and medicated with furosemide was significantly different. Pre-race furosemide significantly influenced ACE activity post-race, while distance raced, temperature, humidity, and haematocrit did not. Horses medicated with pre-race furosemide still demonstrated some degree of bleeding after the race and were at higher risk of presenting EIPH than non-medicated horses. Horses medicated with furosemide have lower circulating ACE activity which might indicate a protective effect of furosemide. Furosemide might reduce EIPH severity after a single bout of exercise, but it does not abolish or reduce its occurrence.

Original languageEnglish
Pages (from-to)119-125
Number of pages7
JournalComparative Exercise Physiology
Volume14
Issue number2
DOIs
Publication statusPublished - 2018 Jan 1

Fingerprint

furosemide
peptidyl-dipeptidase A
Furosemide
Enzyme activity
Peptidyl-Dipeptidase A
Horses
enzyme activity
hemorrhage
exercise
Exercise
Hemorrhage
horses
lungs
Lung
respiratory tract endoscopy
Linear Models
vasoconstrictor agents
Animal Welfare
Endoscopy
racehorses

Keywords

  • Biomarkers
  • Exercise induced pulmonary haemorrhage
  • Horse

ASJC Scopus subject areas

  • Biophysics
  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Physiology
  • Orthopedics and Sports Medicine
  • veterinary (miscalleneous)
  • Physiology (medical)

Cite this

De Mello Costa, M. F., Ronchi, F. A., Jung, Y., Ivanow, A., Braga, J. V., Ramos, M. T., ... Slocombe, R. F. (2018). ACE activity post-race is influenced by furosemide administration. Comparative Exercise Physiology, 14(2), 119-125. https://doi.org/10.3920/CEP170032

ACE activity post-race is influenced by furosemide administration. / De Mello Costa, M. F.; Ronchi, F. A.; Jung, Yoonsuh; Ivanow, A.; Braga, J. V.; Ramos, M. T.; Casarini, D. E.; Slocombe, R. F.

In: Comparative Exercise Physiology, Vol. 14, No. 2, 01.01.2018, p. 119-125.

Research output: Contribution to journalArticle

De Mello Costa, MF, Ronchi, FA, Jung, Y, Ivanow, A, Braga, JV, Ramos, MT, Casarini, DE & Slocombe, RF 2018, 'ACE activity post-race is influenced by furosemide administration', Comparative Exercise Physiology, vol. 14, no. 2, pp. 119-125. https://doi.org/10.3920/CEP170032
De Mello Costa MF, Ronchi FA, Jung Y, Ivanow A, Braga JV, Ramos MT et al. ACE activity post-race is influenced by furosemide administration. Comparative Exercise Physiology. 2018 Jan 1;14(2):119-125. https://doi.org/10.3920/CEP170032
De Mello Costa, M. F. ; Ronchi, F. A. ; Jung, Yoonsuh ; Ivanow, A. ; Braga, J. V. ; Ramos, M. T. ; Casarini, D. E. ; Slocombe, R. F. / ACE activity post-race is influenced by furosemide administration. In: Comparative Exercise Physiology. 2018 ; Vol. 14, No. 2. pp. 119-125.
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