Abstract
The acetate-assisted cultivation of Eubacterium limosum KIST612 was found to provide a way for enhancing cell mass, the carbon monoxide (CO) consumption rate, and butyrate production using CO as an electron and energy source. Cell growth (146%), μmax (121%), and CO consumption rates (151%) increased significantly upon the addition of 30 mM acetate to microbial cultures. The main product of CO fermentation by E. limosum KIST612 shifted from acetate to butyrate in the presence of acetate, and 5.72 mM butyrate was produced at the end of the reaction. The resting cell experimental conditions indicated acetate uptake and an increase in the butyrate concentration. Three routes to acetate assimilation and energy conservation were suggested based on given experimental results and previously genome sequencing data. Acetate assimilation via propionate CoA-transferase (PCT) was expected to produce 1.5 mol ATP/mol butyrate, and was thus anticipated to be the most preferred route.
| Original language | English |
|---|---|
| Pages (from-to) | 560-566 |
| Number of pages | 7 |
| Journal | Bioresource Technology |
| Volume | 245 |
| DOIs | |
| Publication status | Published - 2017 Dec 1 |
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Keywords
- Assimilation of acetate
- Carbon monoxide fermentation
- Eubacterium limosum KIST612
- Product shift
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Waste Management and Disposal
Cite this
Acetate-assisted increase of butyrate production by Eubacterium limosum KIST612 during carbon monoxide fermentation. / Park, Shinyoung; Yasin, Muhammad; Jeong, Jiyeong; Cha, Minseok; Kang, Hyunsoo; Jang, Nulee; Choi, In-Geol; Chang, In Seop.
In: Bioresource Technology, Vol. 245, 01.12.2017, p. 560-566.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Acetate-assisted increase of butyrate production by Eubacterium limosum KIST612 during carbon monoxide fermentation
AU - Park, Shinyoung
AU - Yasin, Muhammad
AU - Jeong, Jiyeong
AU - Cha, Minseok
AU - Kang, Hyunsoo
AU - Jang, Nulee
AU - Choi, In-Geol
AU - Chang, In Seop
PY - 2017/12/1
Y1 - 2017/12/1
N2 - The acetate-assisted cultivation of Eubacterium limosum KIST612 was found to provide a way for enhancing cell mass, the carbon monoxide (CO) consumption rate, and butyrate production using CO as an electron and energy source. Cell growth (146%), μmax (121%), and CO consumption rates (151%) increased significantly upon the addition of 30 mM acetate to microbial cultures. The main product of CO fermentation by E. limosum KIST612 shifted from acetate to butyrate in the presence of acetate, and 5.72 mM butyrate was produced at the end of the reaction. The resting cell experimental conditions indicated acetate uptake and an increase in the butyrate concentration. Three routes to acetate assimilation and energy conservation were suggested based on given experimental results and previously genome sequencing data. Acetate assimilation via propionate CoA-transferase (PCT) was expected to produce 1.5 mol ATP/mol butyrate, and was thus anticipated to be the most preferred route.
AB - The acetate-assisted cultivation of Eubacterium limosum KIST612 was found to provide a way for enhancing cell mass, the carbon monoxide (CO) consumption rate, and butyrate production using CO as an electron and energy source. Cell growth (146%), μmax (121%), and CO consumption rates (151%) increased significantly upon the addition of 30 mM acetate to microbial cultures. The main product of CO fermentation by E. limosum KIST612 shifted from acetate to butyrate in the presence of acetate, and 5.72 mM butyrate was produced at the end of the reaction. The resting cell experimental conditions indicated acetate uptake and an increase in the butyrate concentration. Three routes to acetate assimilation and energy conservation were suggested based on given experimental results and previously genome sequencing data. Acetate assimilation via propionate CoA-transferase (PCT) was expected to produce 1.5 mol ATP/mol butyrate, and was thus anticipated to be the most preferred route.
KW - Assimilation of acetate
KW - Carbon monoxide fermentation
KW - Eubacterium limosum KIST612
KW - Product shift
UR - http://www.scopus.com/inward/record.url?scp=85028976146&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85028976146&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2017.08.132
DO - 10.1016/j.biortech.2017.08.132
M3 - Article
C2 - 28898856
AN - SCOPUS:85028976146
VL - 245
SP - 560
EP - 566
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -