Acetate-assisted increase of butyrate production by Eubacterium limosum KIST612 during carbon monoxide fermentation

Shinyoung Park, Muhammad Yasin, Jiyeong Jeong, Minseok Cha, Hyunsoo Kang, Nulee Jang, In-Geol Choi, In Seop Chang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The acetate-assisted cultivation of Eubacterium limosum KIST612 was found to provide a way for enhancing cell mass, the carbon monoxide (CO) consumption rate, and butyrate production using CO as an electron and energy source. Cell growth (146%), μmax (121%), and CO consumption rates (151%) increased significantly upon the addition of 30 mM acetate to microbial cultures. The main product of CO fermentation by E. limosum KIST612 shifted from acetate to butyrate in the presence of acetate, and 5.72 mM butyrate was produced at the end of the reaction. The resting cell experimental conditions indicated acetate uptake and an increase in the butyrate concentration. Three routes to acetate assimilation and energy conservation were suggested based on given experimental results and previously genome sequencing data. Acetate assimilation via propionate CoA-transferase (PCT) was expected to produce 1.5 mol ATP/mol butyrate, and was thus anticipated to be the most preferred route.

Original languageEnglish
Pages (from-to)560-566
Number of pages7
JournalBioresource Technology
Volume245
DOIs
Publication statusPublished - 2017 Dec 1

Fingerprint

Butyrates
Carbon Monoxide
carbon monoxide
Carbon monoxide
Fermentation
fermentation
acetate
Acetates
Adenosinetriphosphate
Cell growth
Energy conservation
Genes
energy conservation
Electrons
genome
Adenosine Triphosphate
electron

Keywords

  • Assimilation of acetate
  • Carbon monoxide fermentation
  • Eubacterium limosum KIST612
  • Product shift

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Acetate-assisted increase of butyrate production by Eubacterium limosum KIST612 during carbon monoxide fermentation. / Park, Shinyoung; Yasin, Muhammad; Jeong, Jiyeong; Cha, Minseok; Kang, Hyunsoo; Jang, Nulee; Choi, In-Geol; Chang, In Seop.

In: Bioresource Technology, Vol. 245, 01.12.2017, p. 560-566.

Research output: Contribution to journalArticle

Park, Shinyoung ; Yasin, Muhammad ; Jeong, Jiyeong ; Cha, Minseok ; Kang, Hyunsoo ; Jang, Nulee ; Choi, In-Geol ; Chang, In Seop. / Acetate-assisted increase of butyrate production by Eubacterium limosum KIST612 during carbon monoxide fermentation. In: Bioresource Technology. 2017 ; Vol. 245. pp. 560-566.
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