Acetoin production using metabolically engineered Klebsiella pneumoniae

Ji Woong Jang, Hwi Min Jung, Duck Gyun Kim, Min-Kyu Oh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Acetoin is variously applicable platform chemical in chemical and food industry. In this study, Klebsiella pneumoniae was engineered for acetoin production using metabolic engineering. From the recombinant Klebsiella pneumoniae (KMK-05) producing 2,3-butanediol, budC and dhaD genes encoding two 2,3-butanediol dehydrogenases were deleted to reduce 2,3-butanediol production. Furthermore, a transcriptional regulator, AcoK, was deleted to reduce the expression levels of acetoin degrading enzyme. Lastly, NADH oxidase was overexpressed for adjusting intracellular redox balance. The resulting strain (KJW-03-nox) produced considerable amount of acetoin, with concentration reaching 51 g/L with 2.6 g/L/h maximum productivity in 36 h fed-batch fermentation.

Original languageEnglish
Pages (from-to)237-241
Number of pages5
JournalKorean Chemical Engineering Research
Volume55
Issue number2
DOIs
Publication statusPublished - 2017 Apr 1

Keywords

  • 2 3-Butanediol dehydrogenase
  • Acetoin
  • Metabolic engineering
  • NADH oxidase

ASJC Scopus subject areas

  • Chemical Engineering(all)

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