Acetoin production using metabolically engineered Klebsiella pneumoniae

Ji Woong Jang; Hwi Min Jung; Duck Gyun Kim; Min-Kyu Oh

doi:10.9713/kcer.2017.55.2.237

Acetoin production using metabolically engineered Klebsiella pneumoniae

Ji Woong Jang, Hwi Min Jung, Duck Gyun Kim, Min-Kyu Oh

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Acetoin is variously applicable platform chemical in chemical and food industry. In this study, Klebsiella pneumoniae was engineered for acetoin production using metabolic engineering. From the recombinant Klebsiella pneumoniae (KMK-05) producing 2,3-butanediol, budC and dhaD genes encoding two 2,3-butanediol dehydrogenases were deleted to reduce 2,3-butanediol production. Furthermore, a transcriptional regulator, AcoK, was deleted to reduce the expression levels of acetoin degrading enzyme. Lastly, NADH oxidase was overexpressed for adjusting intracellular redox balance. The resulting strain (KJW-03-nox) produced considerable amount of acetoin, with concentration reaching 51 g/L with 2.6 g/L/h maximum productivity in 36 h fed-batch fermentation.

Original language	English
Pages (from-to)	237-241
Number of pages	5
Journal	Korean Chemical Engineering Research
Volume	55
Issue number	2
DOIs	https://doi.org/10.9713/kcer.2017.55.2.237
Publication status	Published - 2017 Apr 1

Keywords

2 3-Butanediol dehydrogenase
Acetoin
Metabolic engineering
NADH oxidase

ASJC Scopus subject areas

Chemical Engineering(all)

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abstract = "Acetoin is variously applicable platform chemical in chemical and food industry. In this study, Klebsiella pneumoniae was engineered for acetoin production using metabolic engineering. From the recombinant Klebsiella pneumoniae (KMK-05) producing 2,3-butanediol, budC and dhaD genes encoding two 2,3-butanediol dehydrogenases were deleted to reduce 2,3-butanediol production. Furthermore, a transcriptional regulator, AcoK, was deleted to reduce the expression levels of acetoin degrading enzyme. Lastly, NADH oxidase was overexpressed for adjusting intracellular redox balance. The resulting strain (KJW-03-nox) produced considerable amount of acetoin, with concentration reaching 51 g/L with 2.6 g/L/h maximum productivity in 36 h fed-batch fermentation.",

keywords = "2 3-Butanediol dehydrogenase, Acetoin, Metabolic engineering, NADH oxidase",

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