Abstract
Acetoin is variously applicable platform chemical in chemical and food industry. In this study, Klebsiella pneumoniae was engineered for acetoin production using metabolic engineering. From the recombinant Klebsiella pneumoniae (KMK-05) producing 2,3-butanediol, budC and dhaD genes encoding two 2,3-butanediol dehydrogenases were deleted to reduce 2,3-butanediol production. Furthermore, a transcriptional regulator, AcoK, was deleted to reduce the expression levels of acetoin degrading enzyme. Lastly, NADH oxidase was overexpressed for adjusting intracellular redox balance. The resulting strain (KJW-03-nox) produced considerable amount of acetoin, with concentration reaching 51 g/L with 2.6 g/L/h maximum productivity in 36 h fed-batch fermentation.
| Original language | English |
|---|---|
| Pages (from-to) | 237-241 |
| Number of pages | 5 |
| Journal | Korean Chemical Engineering Research |
| Volume | 55 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2017 Apr 1 |
Fingerprint
Keywords
- 2 3-Butanediol dehydrogenase
- Acetoin
- Metabolic engineering
- NADH oxidase
ASJC Scopus subject areas
- Chemical Engineering(all)
Cite this
Acetoin production using metabolically engineered Klebsiella pneumoniae. / Jang, Ji Woong; Jung, Hwi Min; Kim, Duck Gyun; Oh, Min-Kyu.
In: Korean Chemical Engineering Research, Vol. 55, No. 2, 01.04.2017, p. 237-241.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Acetoin production using metabolically engineered Klebsiella pneumoniae
AU - Jang, Ji Woong
AU - Jung, Hwi Min
AU - Kim, Duck Gyun
AU - Oh, Min-Kyu
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Acetoin is variously applicable platform chemical in chemical and food industry. In this study, Klebsiella pneumoniae was engineered for acetoin production using metabolic engineering. From the recombinant Klebsiella pneumoniae (KMK-05) producing 2,3-butanediol, budC and dhaD genes encoding two 2,3-butanediol dehydrogenases were deleted to reduce 2,3-butanediol production. Furthermore, a transcriptional regulator, AcoK, was deleted to reduce the expression levels of acetoin degrading enzyme. Lastly, NADH oxidase was overexpressed for adjusting intracellular redox balance. The resulting strain (KJW-03-nox) produced considerable amount of acetoin, with concentration reaching 51 g/L with 2.6 g/L/h maximum productivity in 36 h fed-batch fermentation.
AB - Acetoin is variously applicable platform chemical in chemical and food industry. In this study, Klebsiella pneumoniae was engineered for acetoin production using metabolic engineering. From the recombinant Klebsiella pneumoniae (KMK-05) producing 2,3-butanediol, budC and dhaD genes encoding two 2,3-butanediol dehydrogenases were deleted to reduce 2,3-butanediol production. Furthermore, a transcriptional regulator, AcoK, was deleted to reduce the expression levels of acetoin degrading enzyme. Lastly, NADH oxidase was overexpressed for adjusting intracellular redox balance. The resulting strain (KJW-03-nox) produced considerable amount of acetoin, with concentration reaching 51 g/L with 2.6 g/L/h maximum productivity in 36 h fed-batch fermentation.
KW - 2 3-Butanediol dehydrogenase
KW - Acetoin
KW - Metabolic engineering
KW - NADH oxidase
UR - http://www.scopus.com/inward/record.url?scp=85016984664&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85016984664&partnerID=8YFLogxK
U2 - 10.9713/kcer.2017.55.2.237
DO - 10.9713/kcer.2017.55.2.237
M3 - Article
AN - SCOPUS:85016984664
VL - 55
SP - 237
EP - 241
JO - Korean Chemical Engineering Research
JF - Korean Chemical Engineering Research
SN - 0304-128X
IS - 2
ER -