TY - JOUR
T1 - Acetoin production using metabolically engineered Klebsiella pneumoniae
AU - Jang, Ji Woong
AU - Jung, Hwi Min
AU - Kim, Duck Gyun
AU - Oh, Min-Kyu
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Acetoin is variously applicable platform chemical in chemical and food industry. In this study, Klebsiella pneumoniae was engineered for acetoin production using metabolic engineering. From the recombinant Klebsiella pneumoniae (KMK-05) producing 2,3-butanediol, budC and dhaD genes encoding two 2,3-butanediol dehydrogenases were deleted to reduce 2,3-butanediol production. Furthermore, a transcriptional regulator, AcoK, was deleted to reduce the expression levels of acetoin degrading enzyme. Lastly, NADH oxidase was overexpressed for adjusting intracellular redox balance. The resulting strain (KJW-03-nox) produced considerable amount of acetoin, with concentration reaching 51 g/L with 2.6 g/L/h maximum productivity in 36 h fed-batch fermentation.
AB - Acetoin is variously applicable platform chemical in chemical and food industry. In this study, Klebsiella pneumoniae was engineered for acetoin production using metabolic engineering. From the recombinant Klebsiella pneumoniae (KMK-05) producing 2,3-butanediol, budC and dhaD genes encoding two 2,3-butanediol dehydrogenases were deleted to reduce 2,3-butanediol production. Furthermore, a transcriptional regulator, AcoK, was deleted to reduce the expression levels of acetoin degrading enzyme. Lastly, NADH oxidase was overexpressed for adjusting intracellular redox balance. The resulting strain (KJW-03-nox) produced considerable amount of acetoin, with concentration reaching 51 g/L with 2.6 g/L/h maximum productivity in 36 h fed-batch fermentation.
KW - 2 3-Butanediol dehydrogenase
KW - Acetoin
KW - Metabolic engineering
KW - NADH oxidase
UR - http://www.scopus.com/inward/record.url?scp=85016984664&partnerID=8YFLogxK
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U2 - 10.9713/kcer.2017.55.2.237
DO - 10.9713/kcer.2017.55.2.237
M3 - Article
AN - SCOPUS:85016984664
VL - 55
SP - 237
EP - 241
JO - Korean Chemical Engineering Research
JF - Korean Chemical Engineering Research
SN - 0304-128X
IS - 2
ER -