Acetylcholine increases Ca 2+ influx by activation of CaMKII in mouse oocytes

Dawon Kang, Chang Gi Hur, Jae-Yong Park, Jaehee Han, Seong Geun Hong

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

IP 3 -induced Ca 2+ release is the primary mechanism that is responsible for acetylcholine (ACh)-induced Ca 2+ oscillation. However, other mechanisms remain to explain intracellular Ca 2+ elevation. We here report that ACh induces Ca 2+ influx via T-type Ca 2+ channel by activation of Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), and the ACh-induced Ca 2+ influx facilitates the generation of Ca 2+ oscillation in the mouse ovulated oocytes (oocytes MII ). ACh increased Ca 2+ current by 50 ± 21%, and produced Ca 2+ oscillation. However, the currents and Ca 2+ peaks were reduced in Ca 2+ -free extracellular medium. ACh failed to activate Ca 2+ current and to produce Ca 2+ oscillation in oocytes pretreated with KN-93, a CaMKII inhibitor. KN-92, an inactive analogue of KN93, and PKC modulators could not prevent the effect of ACh. These results show that ACh increases T-type Ca 2+ current by activation of CaMKII, independent of the PKC pathway, in the mouse oocytes.

Original languageEnglish
Pages (from-to)476-482
Number of pages7
JournalBiochemical and biophysical research communications
Volume360
Issue number2
DOIs
Publication statusPublished - 2007 Aug 24
Externally publishedYes

Fingerprint

Calcium-Calmodulin-Dependent Protein Kinase Type 2
Acetylcholine
Oocytes
Chemical activation
Calcium-Calmodulin-Dependent Protein Kinases
Protein Kinase Inhibitors
Modulators

Keywords

  • Acetylcholine
  • CaMKII
  • Mice
  • Oocytes
  • T-type calcium channel

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Acetylcholine increases Ca 2+ influx by activation of CaMKII in mouse oocytes . / Kang, Dawon; Hur, Chang Gi; Park, Jae-Yong; Han, Jaehee; Hong, Seong Geun.

In: Biochemical and biophysical research communications, Vol. 360, No. 2, 24.08.2007, p. 476-482.

Research output: Contribution to journalArticle

Kang, Dawon ; Hur, Chang Gi ; Park, Jae-Yong ; Han, Jaehee ; Hong, Seong Geun. / Acetylcholine increases Ca 2+ influx by activation of CaMKII in mouse oocytes In: Biochemical and biophysical research communications. 2007 ; Vol. 360, No. 2. pp. 476-482.
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