Acidic cultivation of Haematococcus pluvialis for improved astaxanthin production in the presence of a lethal fungus

Sung Won Hwang, Hong Il Choi, Sang Jun Sim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An acidic cultivation strategy was developed to prevent contamination of a lethal fungus Paraphysoderma sedebokerensis in Haematococcus pluvialis culture for astaxanthin production. Instead of generally used neutral pH, an acidic condition (pH 4) was applied to the cultivation, resulting in a significant inhibition of the fungal contamination. This could be ascribed to the acidity-associated denaturation of a surface protein of P. sedebokerensis, which plays an important role in recognition of H. pluvialis. Stress relief strategies including stepwise light irradiation and naturally occurring nitrogen deficiency were employed in the induction stage to minimize the reduction of astaxanthin production caused by acidic pH. Accordingly, an astaxanthin titer of 84.8 mg L−1 was obtained, which is 141-fold of that from the completely contaminated culture and double of that without the stress relief methods. This strategy provides a persistent contamination control method that can be used for practical astaxanthin production by H. pluvialis.

Original languageEnglish
Pages (from-to)138-144
Number of pages7
JournalBioresource Technology
Volume278
DOIs
Publication statusPublished - 2019 Apr 1

Fingerprint

Fungi
Stress relief
Contamination
fungus
relief
Denaturation
Acidity
acidity
irradiation
Irradiation
fold
Nitrogen
Proteins
Membrane Proteins
protein
nitrogen
astaxanthine
contamination
method

Keywords

  • Acidic cultivation
  • Astaxanthin
  • Haematococcus pluvialis
  • Paraphysoderma sedebokerensis
  • Stress relief

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Acidic cultivation of Haematococcus pluvialis for improved astaxanthin production in the presence of a lethal fungus. / Hwang, Sung Won; Choi, Hong Il; Sim, Sang Jun.

In: Bioresource Technology, Vol. 278, 01.04.2019, p. 138-144.

Research output: Contribution to journalArticle

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