Activation of c-Jun N-terminal kinase antagonizes an anti-apoptotic action of Bcl-2

Jihyun Park, Injung Kim, Young Jun Oh, Ko Woon Lee, Pyung Lim Han, Eui Ju Choi

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Bcl-2 is an intracellular membrane-associated protein that prevents cell death induced by a variety of apoptotic stimuli. A mechanism by which Bcl-2 exerts an anti-cell death effect is, however, not fully understood. In the present study, Bcl-2 suppressed cell death of N18TG neuroglioma cells caused by various apoptotic stresses, including etoposide, staurosporine, anisomycin, and ultraviolet irradiation. Concomitantly, Bcl-2 disrupted a signaling cascade to the c-Jun N-terminal kinase activation induced by the apoptotic stresses. Bcl-2 also prevented the etoposide-induced stimulation of MEKK1. Furthermore, overexpression of c-Jun N-terminal kinase antagonized the death-protective function of Bcl-2. These data suggest that suppression of the c-Jun N-terminal kinase signaling pathway may be critical for Bcl-2 action.

Original languageEnglish
Pages (from-to)16725-16728
Number of pages4
JournalJournal of Biological Chemistry
Volume272
Issue number27
DOIs
Publication statusPublished - 1997 Jul 4

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JNK Mitogen-Activated Protein Kinases
Cell death
Cell Death
Chemical activation
Etoposide
Anisomycin
Intracellular Membranes
Staurosporine
Membrane Proteins
Irradiation

ASJC Scopus subject areas

  • Biochemistry

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Activation of c-Jun N-terminal kinase antagonizes an anti-apoptotic action of Bcl-2. / Park, Jihyun; Kim, Injung; Oh, Young Jun; Lee, Ko Woon; Han, Pyung Lim; Choi, Eui Ju.

In: Journal of Biological Chemistry, Vol. 272, No. 27, 04.07.1997, p. 16725-16728.

Research output: Contribution to journalArticle

Park, Jihyun ; Kim, Injung ; Oh, Young Jun ; Lee, Ko Woon ; Han, Pyung Lim ; Choi, Eui Ju. / Activation of c-Jun N-terminal kinase antagonizes an anti-apoptotic action of Bcl-2. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 27. pp. 16725-16728.
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