Activation of CCL20 and its receptor CCR6 promotes endometrium preparation for implantation and placenta development during the early pregnancy period in pigs

Chanho Park, Hyocheol Bae, Fuller W. Bazer, Gwonhwa Song, Whasun Lim

Research output: Contribution to journalArticle

Abstract

The success of implantation and placentation during early pregnancy depends on the intrauterine environment of diverse histotrophs, including nutrients, growth factors, cytokines, and chemokines. Here, we determined the functional role and signal transduction of CC-motif chemokine 20 (CCL20) in the porcine endometrium during pregnancy. The expression of CCL20 and its receptor CCR6 was abundant in the glandular epithelium (GE) and luminal epithelium (LE) of the porcine endometrium during early pregnancy. Administration of CCL20 to porcine endometrial LE cells increased cellular proliferation with strong PCNA expression by activation of PI3K and MAPK signaling. Blocking PI3K and MAPK decreased the CCL20-mediated elevated proliferation of pLE cells. Moreover, the proliferation of pLE cells was enhanced by inhibiting the LPS-induced cytokines and tunicamycin-induced endoplasmic reticulum (ER) stress response proteins. Overall, these results suggest that CCL20 may improve the endometrial receptivity through inducing proliferative signal transduction in the porcine endometrium during the early gestational period.

Original languageEnglish
Pages (from-to)35-42
Number of pages8
JournalDevelopmental and Comparative Immunology
Volume92
DOIs
Publication statusPublished - 2019 Mar 1

Fingerprint

CCR6 Receptors
Endometrium
Placenta
Swine
Pregnancy
Epithelium
Cell Proliferation
Phosphatidylinositol 3-Kinases
Chemokine CCL20
Signal Transduction
Cytokines
Tunicamycin
Placentation
CC Chemokines
Endoplasmic Reticulum Stress
Proliferating Cell Nuclear Antigen
Heat-Shock Proteins
Chemokines
Intercellular Signaling Peptides and Proteins
Food

Keywords

  • CCL20
  • CCR6
  • Development
  • Endometrium
  • Luminal epithelial cell
  • Porcine

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this

Activation of CCL20 and its receptor CCR6 promotes endometrium preparation for implantation and placenta development during the early pregnancy period in pigs. / Park, Chanho; Bae, Hyocheol; Bazer, Fuller W.; Song, Gwonhwa; Lim, Whasun.

In: Developmental and Comparative Immunology, Vol. 92, 01.03.2019, p. 35-42.

Research output: Contribution to journalArticle

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