Activation of monocytes, T-lymphocytes and plasma inflammatory markers in angina patients

W. H. Lee, Y. Lee, J. R. Kim, Jina Choo, S. Y. Lee, J. O. Jung, J. S. Kim, S. Kim, J. D. Seo, S. S. Rhee, J. E. Park

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Inflammation and activation of immune cells have important roles in the pathogenesis of atherosclerosis. We analyzed the plasma levels of inflammatory markers and the degree of activation of peripheral blood monocytes and T-lymphocytes isolated from 12 unstable angina, 12 stable angina, and 12 normal subjects. In 20%-33% of patients, monocytes expressed high basal levels of IL-8, tissue factor, IL-1β, and monocyte chemoattractant protein-1 mRNA. Furthermore, basal mRNA levels of these cytokines showed strong correlation with each other (p < 0.01 in all combination) but not with tumor necrosis factor-α or transforming growth factor-β1. Plasma level of C-reactive protein was highest in the unstable angina patients (1.63 ± 0.70 mg/l) and lowest in the control subjects (0.22 ± 0.08 mg/l) (P = 0.03). We also observed a high correlation between C-reactive protein level and the occurrence of minor and major coronary events during 6 months of follow-up. Activation status of T-cells, assessed by the percentage of HLA-DR positive cells, was highest in the unstable angina patients (26.8 ± 1.4%) compared with that in the control (14.7 ± 1.2%) (P = 0.0053). Our data represent the first case showing that the circulating monocytes in angina patients are activated to a state express numerous proatherogenic cytokines. These results may help to diagnose angina patients according to the inflammatory markers and evaluate the prognosis of the disease.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalExperimental and Molecular Medicine
Volume31
Issue number3
Publication statusPublished - 1999 Sep 30
Externally publishedYes

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T-cells
Monocytes
Chemical activation
Unstable Angina
T-Lymphocytes
Plasmas
C-Reactive Protein
Cytokines
Messenger RNA
Chemokine CCL2
Thromboplastin
Transforming Growth Factors
HLA-DR Antigens
Interleukin-8
Interleukin-1
Blood
Tumor Necrosis Factor-alpha
Cells
Stable Angina
Atherosclerosis

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Lee, W. H., Lee, Y., Kim, J. R., Choo, J., Lee, S. Y., Jung, J. O., ... Park, J. E. (1999). Activation of monocytes, T-lymphocytes and plasma inflammatory markers in angina patients. Experimental and Molecular Medicine, 31(3), 159-164.

Activation of monocytes, T-lymphocytes and plasma inflammatory markers in angina patients. / Lee, W. H.; Lee, Y.; Kim, J. R.; Choo, Jina; Lee, S. Y.; Jung, J. O.; Kim, J. S.; Kim, S.; Seo, J. D.; Rhee, S. S.; Park, J. E.

In: Experimental and Molecular Medicine, Vol. 31, No. 3, 30.09.1999, p. 159-164.

Research output: Contribution to journalArticle

Lee, WH, Lee, Y, Kim, JR, Choo, J, Lee, SY, Jung, JO, Kim, JS, Kim, S, Seo, JD, Rhee, SS & Park, JE 1999, 'Activation of monocytes, T-lymphocytes and plasma inflammatory markers in angina patients', Experimental and Molecular Medicine, vol. 31, no. 3, pp. 159-164.
Lee, W. H. ; Lee, Y. ; Kim, J. R. ; Choo, Jina ; Lee, S. Y. ; Jung, J. O. ; Kim, J. S. ; Kim, S. ; Seo, J. D. ; Rhee, S. S. ; Park, J. E. / Activation of monocytes, T-lymphocytes and plasma inflammatory markers in angina patients. In: Experimental and Molecular Medicine. 1999 ; Vol. 31, No. 3. pp. 159-164.
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N2 - Inflammation and activation of immune cells have important roles in the pathogenesis of atherosclerosis. We analyzed the plasma levels of inflammatory markers and the degree of activation of peripheral blood monocytes and T-lymphocytes isolated from 12 unstable angina, 12 stable angina, and 12 normal subjects. In 20%-33% of patients, monocytes expressed high basal levels of IL-8, tissue factor, IL-1β, and monocyte chemoattractant protein-1 mRNA. Furthermore, basal mRNA levels of these cytokines showed strong correlation with each other (p < 0.01 in all combination) but not with tumor necrosis factor-α or transforming growth factor-β1. Plasma level of C-reactive protein was highest in the unstable angina patients (1.63 ± 0.70 mg/l) and lowest in the control subjects (0.22 ± 0.08 mg/l) (P = 0.03). We also observed a high correlation between C-reactive protein level and the occurrence of minor and major coronary events during 6 months of follow-up. Activation status of T-cells, assessed by the percentage of HLA-DR positive cells, was highest in the unstable angina patients (26.8 ± 1.4%) compared with that in the control (14.7 ± 1.2%) (P = 0.0053). Our data represent the first case showing that the circulating monocytes in angina patients are activated to a state express numerous proatherogenic cytokines. These results may help to diagnose angina patients according to the inflammatory markers and evaluate the prognosis of the disease.

AB - Inflammation and activation of immune cells have important roles in the pathogenesis of atherosclerosis. We analyzed the plasma levels of inflammatory markers and the degree of activation of peripheral blood monocytes and T-lymphocytes isolated from 12 unstable angina, 12 stable angina, and 12 normal subjects. In 20%-33% of patients, monocytes expressed high basal levels of IL-8, tissue factor, IL-1β, and monocyte chemoattractant protein-1 mRNA. Furthermore, basal mRNA levels of these cytokines showed strong correlation with each other (p < 0.01 in all combination) but not with tumor necrosis factor-α or transforming growth factor-β1. Plasma level of C-reactive protein was highest in the unstable angina patients (1.63 ± 0.70 mg/l) and lowest in the control subjects (0.22 ± 0.08 mg/l) (P = 0.03). We also observed a high correlation between C-reactive protein level and the occurrence of minor and major coronary events during 6 months of follow-up. Activation status of T-cells, assessed by the percentage of HLA-DR positive cells, was highest in the unstable angina patients (26.8 ± 1.4%) compared with that in the control (14.7 ± 1.2%) (P = 0.0053). Our data represent the first case showing that the circulating monocytes in angina patients are activated to a state express numerous proatherogenic cytokines. These results may help to diagnose angina patients according to the inflammatory markers and evaluate the prognosis of the disease.

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