Activation of the intrinsic mitochondrial apoptotic pathway in swine influenza virus-mediated cell death

Ki Choi Young, Tae Kyung Kim, Chul Joong Kim, Joong Seob Lee, Se Young Oh, Soo Joo Han, Douglas N. Foster, Ki Chang Hong, Seungkwon You, Hyunggee Kim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The mitochondrial pathway of swine influenza virus (SIV)-induced apoptosis was investigated using porcine kidney (PK-15) cells, swine testicle (ST) cells, and HeLa cervical carcinoma cells which are known not to support viral replication. As judged by cell morphology, annexin V staining, and DNA fragmentation, PK-15 and ST cells infected with three different subtypes of SIV (H1N1, H3N2, and H1N2) were obviously killed by apoptosis, not necrosis. SIV infection in PK-15 and HeLa cells was shown to decrease the cellular levels of Bcl-2 protein compared to that of mock-infected control cells at 24 h post-infection, whereas expression levels of Bax protein increased in the PK-15 cells, but did not increase in HeLa cells by SIV infection. Cytochrome c upregulation was also observed in cytosolic fractions of the PK-15 and HeLa cells infected with SIV. Apoptosome (a multi-protein complex consisting of cytochrome c, Apaf-1, caspase-9, and ATP) formation was confirmed by immunoprecipitation using cytochrome c antibody. Furthermore, SIV infection increased the cellular levels of TAJ, an activator of the JNK- stressing pathway, and the c-Jun protein in the PK-15 and HeLa cells. Taken together, these results suggest that the mitochondrial pathway should be implicated in the apoptosis of PK-15 cells induced by SIV infection.

Original languageEnglish
Pages (from-to)11-17
Number of pages7
JournalExperimental and Molecular Medicine
Volume38
Issue number1
Publication statusPublished - 2006 Feb 28

Fingerprint

Cell death
Orthomyxoviridae
Viruses
Cell Death
Swine
Chemical activation
HeLa Cells
Cytochromes c
Virus Diseases
Apoptosis
Proto-Oncogene Proteins c-jun
bcl-2-Associated X Protein
Testis
Caspase 9
Annexin A5
Apoptosomes
Cytochromes c1
MAP Kinase Signaling System
Proteins
Adenosine Triphosphate

Keywords

  • Apoptosis
  • Cytochrome c
  • Influenza virus
  • Mitochondria
  • Proto-oncogene proteins c-Bcl-2
  • Proto-oncogene proteins c-jun

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Activation of the intrinsic mitochondrial apoptotic pathway in swine influenza virus-mediated cell death. / Young, Ki Choi; Kim, Tae Kyung; Kim, Chul Joong; Lee, Joong Seob; Oh, Se Young; Han, Soo Joo; Foster, Douglas N.; Hong, Ki Chang; You, Seungkwon; Kim, Hyunggee.

In: Experimental and Molecular Medicine, Vol. 38, No. 1, 28.02.2006, p. 11-17.

Research output: Contribution to journalArticle

Young, Ki Choi ; Kim, Tae Kyung ; Kim, Chul Joong ; Lee, Joong Seob ; Oh, Se Young ; Han, Soo Joo ; Foster, Douglas N. ; Hong, Ki Chang ; You, Seungkwon ; Kim, Hyunggee. / Activation of the intrinsic mitochondrial apoptotic pathway in swine influenza virus-mediated cell death. In: Experimental and Molecular Medicine. 2006 ; Vol. 38, No. 1. pp. 11-17.
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