Activation of toll-like receptor 3 attenuates alcoholic liver injury by stimulating Kupffer cells and stellate cells to produce interleukin-10 in mice

Jin Seok Byun, Yang Gun Suh, Hyon Seung Yi, Young-Sun Lee, Won Il Jeong

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background & Aims: The important function of toll-like receptor (TLR) 4 in Kupffer cells and hepatic stellate cells (HSCs) has been well documented in alcoholic liver injury. However, little is known about the role of TLR3. Thus, we tested whether TLR3 activation in HSCs and Kupffer cells could attenuate alcoholic liver injury in vivo, and investigated its possible mechanism in vitro. Methods: Alcoholic liver injury was achieved by feeding wild type (WT), TLR3 knockout (TLR3-/-) and interleukin (IL)-10-/- mice with high-fat diet plus binge ethanol drinking for 2 weeks. To activate TLR3, polyinosinic-polycytidylic acid (poly I:C) was injected into mice. For in vitro studies, HSCs and Kupffer cells were isolated and treated with poly I:C. Results: In WT mice, poly I:C treatment reduced alcoholic liver injury and fat accumulation by suppressing nuclear factor-κB activation and sterol response element-binding protein 1c expression in the liver. In addition, freshly isolated HSCs and Kupffer cells from poly I:C-treated mice showed enhanced expression of IL-10 compared to controls. Infiltrated macrophage numbers and the expression of tumor necrosis factor-α, monocyte chemoattractant protein-1 and IL-6 on these cells were decreased after poly I:C treatment. In vitro, poly I:C treatment enhanced the expression of IL-10 via a TLR3-dependent mechanism in HSCs and Kupffer cells. Finally, the protective effects of poly I:C on alcoholic liver injury were diminished in TLR3 -/- and IL-10-/- mice. Conclusions: TLR3 activation ameliorates alcoholic liver injury via the stimulation of IL-10 production in HSCs and Kupffer cells. TLR3 could be a novel therapeutic target for the treatment of alcoholic liver injury.

Original languageEnglish
Pages (from-to)342-349
Number of pages8
JournalJournal of Hepatology
Volume58
Issue number2
DOIs
Publication statusPublished - 2013 Feb 1
Externally publishedYes

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Toll-Like Receptor 3
Kupffer Cells
Hepatic Stellate Cells
Interleukin-10
Poly C
Liver
Wounds and Injuries
Poly I-C
Binge Drinking
Toll-Like Receptor 4
Chemokine CCL2
Response Elements
High Fat Diet
Sterols
Interleukin-6
Carrier Proteins
Ethanol
Tumor Necrosis Factor-alpha
Fats
Macrophages

Keywords

  • Endocannabinoid
  • Poly I:C
  • Steatohepatitis
  • Toll-like receptor 4

ASJC Scopus subject areas

  • Hepatology

Cite this

Activation of toll-like receptor 3 attenuates alcoholic liver injury by stimulating Kupffer cells and stellate cells to produce interleukin-10 in mice. / Byun, Jin Seok; Suh, Yang Gun; Yi, Hyon Seung; Lee, Young-Sun; Jeong, Won Il.

In: Journal of Hepatology, Vol. 58, No. 2, 01.02.2013, p. 342-349.

Research output: Contribution to journalArticle

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T1 - Activation of toll-like receptor 3 attenuates alcoholic liver injury by stimulating Kupffer cells and stellate cells to produce interleukin-10 in mice

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AU - Suh, Yang Gun

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AU - Lee, Young-Sun

AU - Jeong, Won Il

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