Activation tagging in Arabidopsis

Detlef Weigel, Ji Hoon Ahn, Miguel A. Blázquez, Justin O. Borevitz, Sioux K. Christensen, Christian Fankhauser, Cristina Ferrándiz, Igor Kardailsky, Elizabeth J. Malancharuvil, Michael M. Neff, Jasmine Thuy Nguyen, Shusei Sato, Zhi Yong Wang, Yiji Xia, Richard A. Dixon, Maria J. Harrison, Chris J. Lamb, Martin F. Yanofsky, Joanne Chory

Research output: Contribution to journalArticlepeer-review

724 Citations (Scopus)

Abstract

Activation tagging using T-DNA vectors that contain multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene has been applied to Arabidopsis plants. New activation-tagging vectors that confer resistance to the antibiotic kanamycin or the herbicide glufosinate have been used to generate several tens of thousands of transformed plants. From these, over 30 dominant mutants with various phenotypes have been isolated. Analysis of a subset of mutants has shown that overexpressed genes are almost always found immediately adjacent to the inserted CaMV 35S enhancers, at distances ranging from 380 bp to 3.6 kb. In at least one case, the CaMV 35S enhancers led primarily to an enhancement of the endogenous expression pattern rather than to constitutive ectopic expression, suggesting that the CaMV 35S enhancers used here act differently than the complete CaMV 35S promoter. This has important implications for the spectrum of genes that will be discovered by this method.

Original languageEnglish
Pages (from-to)1003-1013
Number of pages11
JournalPlant physiology
Volume122
Issue number4
DOIs
Publication statusPublished - 2000 Apr

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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