Active DNA topoisomerase II with minimum molecular mass from regenerating rat liver

Se-Ho Park, C. G. Lee, R. Namkung, C. G. Kim, S. D. Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have purified the type II DNA topoisomerase from regenerating rat liver. The purified topoisomerase II migrated as two bands with molecular masses of 70 kDa and 55 kDa on SDS-PAGE. Immunoblotting analysis using antiserum against rat topoisomerase II gene product expressed in Escherichia coli suggested that the two bands on SDS-gel are proteolytic products of the intact 173 kDa form. However, these products retained the enzyme activities such as catenation and relaxation of supercoiled circular duplex monomer DNA and unknotting of knotted phage P4 DNA. These results suggest that DNA topoisomerase II consists of different functional domains and that the whole enzyme is not required for its activity. The activities of the purified enzyme were completely inhibited by 1 mM novobiocin, a bacterial gyrase inhibitor. However, no inhibitory effect was observed when another gyrase inhibitor, nalidixic acid was used.

Original languageEnglish
Pages (from-to)641-650
Number of pages10
JournalBiochemistry and Molecular Biology International
Volume32
Issue number4
Publication statusPublished - 1994 Jan 1
Externally publishedYes

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Type II DNA Topoisomerase
Molecular mass
Liver
Rats
Enzymes
Novobiocin
Nalidixic Acid
Bacteriophages
DNA
Enzyme activity
Immunoblotting
Escherichia coli
Immune Sera
Polyacrylamide Gel Electrophoresis
Genes
Monomers
Gels

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Active DNA topoisomerase II with minimum molecular mass from regenerating rat liver. / Park, Se-Ho; Lee, C. G.; Namkung, R.; Kim, C. G.; Park, S. D.

In: Biochemistry and Molecular Biology International, Vol. 32, No. 4, 01.01.1994, p. 641-650.

Research output: Contribution to journalArticle

Park, Se-Ho ; Lee, C. G. ; Namkung, R. ; Kim, C. G. ; Park, S. D. / Active DNA topoisomerase II with minimum molecular mass from regenerating rat liver. In: Biochemistry and Molecular Biology International. 1994 ; Vol. 32, No. 4. pp. 641-650.
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