Active human ferritin H/L-hybrid and sequence effect on folding efficiency in Escherichia coli

Jeewon Lee, Sung Woo Kim, Yang Hoon Kim, Ji Young Ahn

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In Escherichia coli, the recombinant human L-chain ferritin was synthesized in the form of inclusion bodies under the control of T7 promoter system. We developed a recombinant ferritin H/L-hybrid by a direct gene fusion between H- and L-chain subunits. Surprisingly, the presence of heavy-chain polypeptide at the amino terminus of light chain significantly increased the cytoplasmic solubility of the recombinant ferritin hybrid, i.e., more than 80% of synthesized ferritin hybrid was soluble in intracellular region regardless of the changes in cell growth and gene expression conditions such as type of inducer, growth media, culture scale, etc. The soluble ferritin H/L-hybrid was biologically active with the iron storage capacity (295 mol Fe +3 per mol H/L-hybrid) equivalent to ferritin standard. Different types of hybrid mutants were also developed using various H-chain derivatives. Comparison of the intracellular solubilities of the synthesized hybrid mutants showed that the N-terminus four helices of heavy subunit were of crucial importance in maintaining the high solubility in E. coli cytoplasm. Consequently, the increased solubility of the ferritin hybrid seems to be related to such H-chain sequence that forms ferroxidase center and promotes effective intra-molecular interaction with L-chain domain of H/L-hybrid for enhancing the folding efficiency.

Original languageEnglish
Pages (from-to)225-229
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume298
Issue number2
DOIs
Publication statusPublished - 2002 Nov 18

Fingerprint

Apoferritins
Ferritins
Escherichia coli
Solubility
Ceruloplasmin
Molecular interactions
Cell growth
Gene expression
Culture Media
Fusion reactions
Iron
Genes
Derivatives
Gene Fusion
Inclusion Bodies
Peptides
Growth
Cytoplasm
Gene Expression
Light

Keywords

  • Cytoplasmic solubility
  • Ferritin H/L-hybrid
  • Folding efficiency
  • Intra-molecular interaction
  • Iron storage capacity

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Active human ferritin H/L-hybrid and sequence effect on folding efficiency in Escherichia coli. / Lee, Jeewon; Kim, Sung Woo; Kim, Yang Hoon; Ahn, Ji Young.

In: Biochemical and Biophysical Research Communications, Vol. 298, No. 2, 18.11.2002, p. 225-229.

Research output: Contribution to journalArticle

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