Acute effect of high-dose isoflavones from Pueraria lobata (willd.) Ohwi on lipid and bone metabolism in ovariectomized mice

Hee Joon Cho, Hee Jin Jun, Ji Hae Lee, Yaoyao Jia, Minh Hien Hoang, Jae Hoon Shim, Kwan Hwa Park, Sung-Joon Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We investigated the acute metabolic effects of isoflavones from Pueraria lobata (Willd.) Ohwi (IPL) in ovariectomized (OVX) mice. After 4 weeks of IPL feeding at 500 mg/day/kg body weight (OVX500), plasma 17β-estradiol concentrations were significantly higher (+25%, p < 0.05), whereas plasma triglyceride levels were significantly lower in OVX mice (-15%, p < 0.05) compared with controls. Abdominal adipose tissue weight was marginally reduced in IPL-fed groups compared with OVX controls and the plasma levels of liver enzymes were unchanged. In addition, IPL significantly inhibited the reduction of bone mineral density in the femurs of OVX mice (OVX200, +22%; OVX500, +26%; p < 0.05) compared with controls after 4 weeks of IPL feeding. In quantitative polymerase chain reaction analysis the expression of aromatase was significantly suppressed and SULT1E1 was increased by IPL feeding, showing that IPL feeding may not alter the risk for breast cancer in mice. Our results suggest that IPL could ameliorate menopausal symptoms in mice. Further studies will confirm the effects of IPL in humans.

Original languageEnglish
Pages (from-to)1864-1871
Number of pages8
JournalPhytotherapy Research
Volume26
Issue number12
DOIs
Publication statusPublished - 2012 Dec 1

Fingerprint

Lipid Metabolism
Bone and Bones
Abdominal Fat
Aromatase
Bone Density
Femur
Estradiol
Triglycerides
Body Weight
yufengningxin
Breast Neoplasms
Weights and Measures
Polymerase Chain Reaction
Liver
Enzymes

Keywords

  • bone
  • lipid metabolism
  • ovariectomy
  • Pueraria lobata (Willd.) Ohwi
  • puerarin

ASJC Scopus subject areas

  • Pharmacology

Cite this

Acute effect of high-dose isoflavones from Pueraria lobata (willd.) Ohwi on lipid and bone metabolism in ovariectomized mice. / Cho, Hee Joon; Jun, Hee Jin; Lee, Ji Hae; Jia, Yaoyao; Hoang, Minh Hien; Shim, Jae Hoon; Park, Kwan Hwa; Lee, Sung-Joon.

In: Phytotherapy Research, Vol. 26, No. 12, 01.12.2012, p. 1864-1871.

Research output: Contribution to journalArticle

Cho, Hee Joon ; Jun, Hee Jin ; Lee, Ji Hae ; Jia, Yaoyao ; Hoang, Minh Hien ; Shim, Jae Hoon ; Park, Kwan Hwa ; Lee, Sung-Joon. / Acute effect of high-dose isoflavones from Pueraria lobata (willd.) Ohwi on lipid and bone metabolism in ovariectomized mice. In: Phytotherapy Research. 2012 ; Vol. 26, No. 12. pp. 1864-1871.
@article{f0aa90f4b6934dff8bd22b9255ac7e6a,
title = "Acute effect of high-dose isoflavones from Pueraria lobata (willd.) Ohwi on lipid and bone metabolism in ovariectomized mice",
abstract = "We investigated the acute metabolic effects of isoflavones from Pueraria lobata (Willd.) Ohwi (IPL) in ovariectomized (OVX) mice. After 4 weeks of IPL feeding at 500 mg/day/kg body weight (OVX500), plasma 17β-estradiol concentrations were significantly higher (+25{\%}, p < 0.05), whereas plasma triglyceride levels were significantly lower in OVX mice (-15{\%}, p < 0.05) compared with controls. Abdominal adipose tissue weight was marginally reduced in IPL-fed groups compared with OVX controls and the plasma levels of liver enzymes were unchanged. In addition, IPL significantly inhibited the reduction of bone mineral density in the femurs of OVX mice (OVX200, +22{\%}; OVX500, +26{\%}; p < 0.05) compared with controls after 4 weeks of IPL feeding. In quantitative polymerase chain reaction analysis the expression of aromatase was significantly suppressed and SULT1E1 was increased by IPL feeding, showing that IPL feeding may not alter the risk for breast cancer in mice. Our results suggest that IPL could ameliorate menopausal symptoms in mice. Further studies will confirm the effects of IPL in humans.",
keywords = "bone, lipid metabolism, ovariectomy, Pueraria lobata (Willd.) Ohwi, puerarin",
author = "Cho, {Hee Joon} and Jun, {Hee Jin} and Lee, {Ji Hae} and Yaoyao Jia and Hoang, {Minh Hien} and Shim, {Jae Hoon} and Park, {Kwan Hwa} and Sung-Joon Lee",
year = "2012",
month = "12",
day = "1",
doi = "10.1002/ptr.4669",
language = "English",
volume = "26",
pages = "1864--1871",
journal = "Phytotherapy Research",
issn = "0951-418X",
publisher = "John Wiley and Sons Ltd",
number = "12",

}

TY - JOUR

T1 - Acute effect of high-dose isoflavones from Pueraria lobata (willd.) Ohwi on lipid and bone metabolism in ovariectomized mice

AU - Cho, Hee Joon

AU - Jun, Hee Jin

AU - Lee, Ji Hae

AU - Jia, Yaoyao

AU - Hoang, Minh Hien

AU - Shim, Jae Hoon

AU - Park, Kwan Hwa

AU - Lee, Sung-Joon

PY - 2012/12/1

Y1 - 2012/12/1

N2 - We investigated the acute metabolic effects of isoflavones from Pueraria lobata (Willd.) Ohwi (IPL) in ovariectomized (OVX) mice. After 4 weeks of IPL feeding at 500 mg/day/kg body weight (OVX500), plasma 17β-estradiol concentrations were significantly higher (+25%, p < 0.05), whereas plasma triglyceride levels were significantly lower in OVX mice (-15%, p < 0.05) compared with controls. Abdominal adipose tissue weight was marginally reduced in IPL-fed groups compared with OVX controls and the plasma levels of liver enzymes were unchanged. In addition, IPL significantly inhibited the reduction of bone mineral density in the femurs of OVX mice (OVX200, +22%; OVX500, +26%; p < 0.05) compared with controls after 4 weeks of IPL feeding. In quantitative polymerase chain reaction analysis the expression of aromatase was significantly suppressed and SULT1E1 was increased by IPL feeding, showing that IPL feeding may not alter the risk for breast cancer in mice. Our results suggest that IPL could ameliorate menopausal symptoms in mice. Further studies will confirm the effects of IPL in humans.

AB - We investigated the acute metabolic effects of isoflavones from Pueraria lobata (Willd.) Ohwi (IPL) in ovariectomized (OVX) mice. After 4 weeks of IPL feeding at 500 mg/day/kg body weight (OVX500), plasma 17β-estradiol concentrations were significantly higher (+25%, p < 0.05), whereas plasma triglyceride levels were significantly lower in OVX mice (-15%, p < 0.05) compared with controls. Abdominal adipose tissue weight was marginally reduced in IPL-fed groups compared with OVX controls and the plasma levels of liver enzymes were unchanged. In addition, IPL significantly inhibited the reduction of bone mineral density in the femurs of OVX mice (OVX200, +22%; OVX500, +26%; p < 0.05) compared with controls after 4 weeks of IPL feeding. In quantitative polymerase chain reaction analysis the expression of aromatase was significantly suppressed and SULT1E1 was increased by IPL feeding, showing that IPL feeding may not alter the risk for breast cancer in mice. Our results suggest that IPL could ameliorate menopausal symptoms in mice. Further studies will confirm the effects of IPL in humans.

KW - bone

KW - lipid metabolism

KW - ovariectomy

KW - Pueraria lobata (Willd.) Ohwi

KW - puerarin

UR - http://www.scopus.com/inward/record.url?scp=84871323113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871323113&partnerID=8YFLogxK

U2 - 10.1002/ptr.4669

DO - 10.1002/ptr.4669

M3 - Article

C2 - 22422661

AN - SCOPUS:84871323113

VL - 26

SP - 1864

EP - 1871

JO - Phytotherapy Research

JF - Phytotherapy Research

SN - 0951-418X

IS - 12

ER -